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Moria ultra fine forceps

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The Moria ultra fine forceps are a precision instrument designed for delicate handling and manipulation of small objects. The forceps feature ultra-fine tips for intricate work, allowing users to grasp and control minute samples or specimens with exceptional dexterity and control.

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Lab products found in correlation

Papain dissociation system, by Worthington (3 mentions) Vibratome, by Leica (2 mentions) Hiseq 4000 sequencing platform, by Illumina (1 mentions) Agilent bioanalyzer 2100 system, by Agilent Technologies (1 mentions) Chromium single cell 3 system, by 10x Genomics (1 mentions) Dumont 5 fine forceps, by Fine Science Tools (1 mentions)

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Fine forceps (8 citations)

5 protocols using moria ultra fine forceps

1

Multimodal Profiling of Mouse Embryonic Brain

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Mouse embryonic brain structures were dissected from C57BL6 mice at embryonic timepoints E10.5, E13.5, E16.5, and E18.5. Both male and female mice were used. For the brainstem, an incision was made between the midbrain and hindbrain boundary, as well as between the medullary hindbrain and spinal cord, to isolate rhombomeres 1 to 11 except for the cerebellar structure that was removed. All mouse dissections were performed under a Leica stereoscope with a pair of Moria ultra fine forceps (Fine Science Tools) in a PBS solution. The tissue was transferred into ice-cold Leibovitz’s medium, followed by single-cell dissociation with the Papain Dissociation System (Worthington Biochemical Corporation). Approximately 10,000 cells per sample were loaded on the Chromium Single Cell 3′ system (10x Genomics). GEM-RT, DynaBeads cleanup, PCR amplification and SPRIselect beads cleanup were performed using Chromium Single Cell 3′ Gel Bead kit. Indexed single-cell libraries were generated using the Chromium Single Cell 3′ Library kit and the Chromium i7 Multiplex kit. Size, quality, concentration and purity of the complementary DNAs and the corresponding 10x library were evaluated by the Agilent 2100 Bioanalyzer system. The 10x libraries were sequenced (multiplexed) on the Illumina HiSeq 4000 sequencing platform.
Jessa S., Mohammadnia A., Harutyunyan A.S., Hulswit M., Varadharajan S., Lakkis H., Kabir N., Bashardanesh Z., Hébert S., Faury D., Vladoiu M.C., Worme S., Coutelier M., Krug B., Andrade A.F., Pathania M., Bajic A., Weil A.G., Ellezam B., Atkinson J., Dudley R.W., Farmer J.P., Perreault S., Garcia B.A., Larouche V., Blanchette M., Garzia L., Bhaduri A., Ligon K.L., Bandopadhayay P., Taylor M.D., Mack S.C., Jabado N, & Kleinman C.L. (2022). K27M in canonical and noncanonical H3 variants occurs in distinct oligodendroglial cell lineages in brain midline gliomas. Nature genetics, 54(12), 1865-1880.
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2

Mouse Mammary Gland Intraductal Injection

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Instruments for mouse mammary gland intraductal injections were procured from the following vendors: 100μl capacity Hamilton syringe, with 26-gauge, 0.5-inch, 30o-angle, blunt-ended needle (Hamilton, cat # 80608); 1-ml BD slip-tip tuberculin syringe with 26.5 gauge needle (Thermos Fisher Scientific, Waltham, MA; cat # 309659); small scissors (Fine Science Tools, Foster City, CA; cat# 14028–10); Bonn artery scissors with a ball tip (Fine Science Tools, Foster City, CA; cat# 14086–09); micro-serrated Vannas spring scissors (Fine Science Tools, Foster City, CA; cat# 15007–08); Moria ultra fine forceps (Fine Science Tools, Foster City, CA; cat# 11370–40); Dumont #5—fine forceps (Fine Science Tools, Foster City, CA; cat# 11254–20); Dumont #7—fine forceps (Fine Science Tools, Foster City, CA; cat# 11274–20); delicate suture tying forceps (Fine Science Tools, Foster City, CA; cat# 11063–07); tissue forceps with 1x2 teeth (Fine Science Tools, Foster City, CA; cat# 11021–12); an auto clip system for wound closure with a 9mm clip applier (Fine Science Tools, Foster City, CA; cat# 12020–09); clip remover (Fine Science Tools, Foster City, CA; cat# 12023–00); and clips (Fine Science Tools, Foster City, CA; cat# 12022–09). As a part of post-operative care, an XpressHeat Pad (Sunbeam, Boca Raton, FL; cat# 002013-912-000) was used to maintain body temperature in the mouse.
Ghosh A., Sarkar S., Banerjee S., Behbod F., Tawfik O., McGregor D., Graff S, & Banerjee S.K. (2018). MIND model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis. PLoS ONE, 13(5), e0198143.
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3

Cerebellar Development Tissue Isolation

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Fresh tumor tissue was collected at the time of resection. The tumor tissue was mechanically and enzymatically dissociated using a collagenase-based dissociation method as previously reported41 . Early embryonic hindbrain structures were dissected from the gestational time points E10 and E12. An incision was made between the midbrain and hindbrain boundary, as well as between the prepontine hindbrain and pontine hindbrain, in order to isolate the isthmus, and rhombomeres −1 and −2 at these early time points in development. Late embryonic cerebellar primordia were collected at embryonic time points 14, 16 and 18. All embryonic mouse dissections were performed under a Leica stereoscope with a pair of Moria ultra fine forceps (Fine Science Tools). The tissue was transferred into ice cold Leibovitz’s medium, followed by single cell dissociation with the Papain Dissociation System (Worthington Biochemical Corporation). Postnatal cerebella were dissected from the following time points: day 0, 5, 7 and 14. The central nervous system was fully dissected, then embedded in 2% Low melting point agarose. One mid sagittal slice of 300 um was generated using the Leica vibratome42 . Under the stereoscope the cerebellum was isolated from the slice, followed by immediate single cell dissociation as described above.
Vladoiu M.C., El-Hamamy I., Donovan L.K., Farooq H., Holgado B.L., Sundaravadanam Y., Ramaswamy V., Hendrikse L.D., Kumar S., Mack S.C., Lee J.J., Fong V., Juraschka K., Przelicki D., Michealraj A., Skowron P., Luu B., Suzuki H., Morrissy A.S., Cavalli F.M., Garzia L., Daniels C., Wu X., Qazi M.A., Singh S.K., Chan J.A., Marra M.A., Malkin D., Dirks P., Heisler L., Pugh T., Ng K., Notta F., Thompson E.M., Kleinman C.L., Joyner A.L., Jabado N., Stein L, & Taylor M.D. (2019). Childhood Cerebellar Tumors Mirror Conserved Fetal Transcriptional Programs. Nature, 572(7767), 67-73.
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4

Cerebellar Development Tissue Isolation

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Fresh tumor tissue was collected at the time of resection. The tumor tissue was mechanically and enzymatically dissociated using a collagenase-based dissociation method as previously reported41 . Early embryonic hindbrain structures were dissected from the gestational time points E10 and E12. An incision was made between the midbrain and hindbrain boundary, as well as between the prepontine hindbrain and pontine hindbrain, in order to isolate the isthmus, and rhombomeres −1 and −2 at these early time points in development. Late embryonic cerebellar primordia were collected at embryonic time points 14, 16 and 18. All embryonic mouse dissections were performed under a Leica stereoscope with a pair of Moria ultra fine forceps (Fine Science Tools). The tissue was transferred into ice cold Leibovitz’s medium, followed by single cell dissociation with the Papain Dissociation System (Worthington Biochemical Corporation). Postnatal cerebella were dissected from the following time points: day 0, 5, 7 and 14. The central nervous system was fully dissected, then embedded in 2% Low melting point agarose. One mid sagittal slice of 300 um was generated using the Leica vibratome42 . Under the stereoscope the cerebellum was isolated from the slice, followed by immediate single cell dissociation as described above.
Vladoiu M.C., El-Hamamy I., Donovan L.K., Farooq H., Holgado B.L., Sundaravadanam Y., Ramaswamy V., Hendrikse L.D., Kumar S., Mack S.C., Lee J.J., Fong V., Juraschka K., Przelicki D., Michealraj A., Skowron P., Luu B., Suzuki H., Morrissy A.S., Cavalli F.M., Garzia L., Daniels C., Wu X., Qazi M.A., Singh S.K., Chan J.A., Marra M.A., Malkin D., Dirks P., Heisler L., Pugh T., Ng K., Notta F., Thompson E.M., Kleinman C.L., Joyner A.L., Jabado N., Stein L, & Taylor M.D. (2019). Childhood Cerebellar Tumors Mirror Conserved Fetal Transcriptional Programs. Nature, 572(7767), 67-73.
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5

Sciatic Nerve Crush Injury Model

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Mice were deeply anesthetized with 2-3% isoflurane mixed with oxygen and the surgical site was shaved and disinfected. A small incision at the level of the mid-thigh was made through the skin and overlying musculature to expose the sciatic nerve. The exposed nerve was then crushed by applying pressure with Moria Ultra Fine Forceps (#11399-80, Fine Science Tools). Following the crush procedure, the muscle was closed with a suture and the skin was closed with staples. In a small number of experiments, a nerve transection was performed instead of a nerve crush. In these instances, the same procedure was performed except that a 3-5mm piece of nerve was excised as opposed to being crushed with forceps.
Shadrach J.L., Stansberry W.M., Milen A.M., Ives R.E., Fogarty E.A., Antonellis A, & Pierchala B.A. (2021). Translatomic analysis of regenerating and degenerating spinal motor neurons in injury and ALS. iScience, 24(7), 102700.
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