Atf3 c19

Manufactured by Santa Cruz Biotechnology

Sourced in United States

ATF3 (C19) is an antibody product offered by Santa Cruz Biotechnology. It is a polyclonal antibody raised against a peptide mapping within the C-terminal domain of ATF3 of human origin. ATF3 is a transcription factor that plays a role in cellular stress response pathways.

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Lab products found in correlation

Lumiglo substrate, by Cell Signaling Technology (2 mentions) Hybond ecl, by GE Healthcare (2 mentions) Hybond, by Cytiva (2 mentions) P ire1α ser724 nb100 2323, by Novus Biologicals (1 mentions) Noxa 114c307.1, by Thermo Fisher Scientific (1 mentions) Necrostatin 1, by Cayman Chemical (1 mentions) Apto 253, by MedChemExpress (1 mentions) Hydroxychloroquine hcq, by Cayman Chemical (1 mentions) α tubulin dm1a, by Merck Group (1 mentions) β actin ac 74, by Merck Group (1 mentions) Ferrostatin 1, by Cayman Chemical (1 mentions) Mcl 1 s 19, by Santa Cruz Biotechnology (1 mentions) Cleaved caspase 3 5a1e, by Cell Signaling Technology (1 mentions) Creb 48h2, by Cell Signaling Technology (1 mentions) Gadd153 f 168, by Santa Cruz Biotechnology (1 mentions) Immpress peroxidase detection reagents, by Vector Laboratories (1 mentions) Vector vip peroxidase substrate kit, by Vector Laboratories (1 mentions) Dab peroxidase substrate kit, by Vector Laboratories (1 mentions) Beta actin ab8227, by Abcam (1 mentions) Chop l63f7, by Cell Signaling Technology (1 mentions)

4 protocols using atf3 c19

Western Blot Analysis of UPR Proteins

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Protein lysates were prepared using RIPA buffer containing 1 mM PMSF, 1 mM sodium orthovanadate, 1 mM NaF, and 30 µL/mL aprotinin. The proteins (20–30 µg) were resolved in SDS-PAGE, electro-transferred onto nitrocellulose membranes (Hybond-ECL, GE Healthcare) and blocked with 5% BSA. Primary antibodies included NRF2 (D1Z9C), BiP/GRP78 (C50B12), IRE1α (14C10), CHOP (L63F7), Calnexin (C5C9) and PERK (D11A8) from Cell Signaling Technologies; p-IRE1α (Ser724; NB100-2323) from Novus Biologicals; GCLC (Ab41463), beta-actin (Ab8227), TBP (ab818) and ATF6 (Ab37149) from Abcam; p-PERK (Thr 981, sc-32577), NQO1 (C-19), and ATF3 (C19) from Santa Cruz. After secondary antibody incubation (1:3000 in TBS-T, 2 h), the proteins were detected using Lumiglo substrate (Cell Signaling Technology, CA) and X-ray films. Immunoblot images are representative of three independent experiments.

Zanotto-Filho A., Masamsetti V.P., Loranc E., Tonapi S.S., Gorthi A., Bernard X., Gonçalves R.M., Moreira J.C., Chen Y, & Bishop A.J. (2016). Alkylating agent induced NRF2 blocks endoplasmic reticulum stress-mediated apoptosis via control of glutathione pools and protein thiol homeostasis. Molecular cancer therapeutics, 15(12), 3000-3014.

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Antibody Acquisition and Small Molecule Procurement

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Antibodies were purchased as follows: BIM (C34C5), BCL-XL (54H6), CREB (48H2) and cleaved caspase-3 (5A1E) from Cell Signaling Technology (Danvers, MA, USA); NOXA (114C307.1) from Thermo Fisher Scientific (Waltham, MA, USA); ATF-3 (C-19) and MCL-1 (S-19) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); KLF4 (56CT5.1.6) from Bioss, Inc. (Woburn, MA, USA); α-tubulin (DM1A) and β-actin (AC-74) from Sigma-Aldrich (Tokyo, Japan). APTO-253 was purchased from MedChemExpress, LLC (Monmouth Junction, NJ, USA). Q-VD-OPh, Doxorubicin, etoposide, SP600125, SB203580, PD184352, and U0126 were purchased from Calbiochem (San Diego, CA, USA). Hydroxychloroquine (HCQ), Necrostatin-1 and Ferrostatin-1 were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Nakajima W., Miyazaki K., Asano Y., Kubota S, & Tanaka N. (2021). Krüppel-Like Factor 4 and Its Activator APTO-253 Induce NOXA-Mediated, p53-Independent Apoptosis in Triple-Negative Breast Cancer Cells. Genes, 12(4), 539.

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Immunohistochemistry Assay for Protein Detection

Cited 1 time

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Immunohistochemistry was performed as described before [5 (link)]. Immunohistochemistry (IHC) was performed using ImmPRESS Peroxidase Detection Reagents (Vector Laboratories) and antibodies specific for HBs (20-HR20, Fitzgerald), GADD153 (F-168, Santa Cruz, Cytokeratin 19 (M-17, Santa Cruz), LHB [26 (link)], ATF-3 (C19, Santa Cruz). Colour reaction was developed with VECTOR VIP Peroxidase Substrate Kit or DAB Peroxidase Substrate Kit, (Vector Laboratories).

Graumann F., Churin Y., Tschuschner A., Reifenberg K., Glebe D., Roderfeld M, & Roeb E. (2015). Genomic Methylation Inhibits Expression of Hepatitis B Virus Envelope Protein in Transgenic Mice: A Non-Infectious Mouse Model to Study Silencing of HBV Surface Antigen Genes. PLoS ONE, 10(12), e0146099.

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Protein Expression Analysis Protocol

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Protein lysates were prepared using RIPA buffer containing 1 mM PMSF, 1 mM sodium orthovanadate, 1 mM NaF, and 30 μL/mL aprotinin. The proteins (20–30 μg) were resolved in SDS-PAGE, electro-transferred onto nitrocellulose membranes (Hybond-ECL, GE Healthcare) and blocked with 5% BSA. Primary antibodies (1:1000 dilution) included NRF2 (D1Z9C), BiP/GRP78 (C50B12) and CHOP (L63F7) from Cell Signaling; beta-actin (ab8227) from Abcam and ATF3 (C19) from Santa Cruz. After secondary antibody incubation (1:3000, 2 h), the proteins were detected using Lumiglo substrate (Cell Signaling Technology, CA).

Zanotto-Filho A., Dashnamoorthy R., Loranc E., de Souza L.H., Moreira J.C., Suresh U., Chen Y, & Bishop A.J. (2016). Combined Gene Expression and RNAi Screening to Identify Alkylation Damage Survival Pathways from Fly to Human. PLoS ONE, 11(4), e0153970.

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