U87MG cells (2 × 105 cells/well) were seeded in 6-well plates for 12 h. TAK901 was then added at 0.31 μM for 72 h. GSC5 cells (2 × 105 cells/well) were seeded and at the same time treated with 0.125 μM TAK901 for 72 h. Cells were fixed in cold 70% ethanol overnight at −20 °C and hydrated for 15 min before staining with a DNA staining solution (Liankebio, Hangzhou, China). The cells were analyzed using a flow cytometer (Beckman Coulter, Brea, CA, USA).
Dna staining solution
Manufactured by Liankebio
Sourced in China
The DNA staining solution is a laboratory reagent used for the detection and visualization of DNA in various applications such as gel electrophoresis, microscopy, and flow cytometry. The solution contains a DNA-binding dye that emits fluorescent light when bound to DNA, allowing for the identification and analysis of DNA samples.
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Lab products found in correlation
4 protocols using dna staining solution
1
Cell Cycle Analysis of Glioblastoma
2
Fibroblast Cell Cycle Analysis
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For cell cycle analysis, fibroblasts were seeded into 6-well plates at a density of 2×10
5‒1×10
6 cells per well. After treatment, cells were harvested, washed with PBS, and incubated with 1 mL DNA staining solution (Lianke, Hangzhou, China) and 10 μL permeabilization solution (Lianke) for 0.5 h at 37°C. The distribution of cell cycle phases was determined with an ACEA NovoExpress Flow Cytometer (Agilent, Santa Clara, USA). The percentages of cells in the G1, S, and G2 phases were calculated.
5‒1×10
6 cells per well. After treatment, cells were harvested, washed with PBS, and incubated with 1 mL DNA staining solution (Lianke, Hangzhou, China) and 10 μL permeabilization solution (Lianke) for 0.5 h at 37°C. The distribution of cell cycle phases was determined with an ACEA NovoExpress Flow Cytometer (Agilent, Santa Clara, USA). The percentages of cells in the G1, S, and G2 phases were calculated.
3
Cell Cycle Analysis of hAT-MSCs
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Flow cytometry was applied for cell cycle analysis. Approximately 2 × 105 hAT-MSCs in each group were harvested and fixed in 75% ethanol for 24 h at − 20 °C, and the cells were then stained with DNA staining solution (LiankeBio, Hangzhou, China) for 30 mins to detect cell cycle distribution.
4
Cell Cycle Analysis by Flow Cytometry
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Flow cytometry was applied for cell cycle analysis. Approximately 2 × 10 5 cells were harvested and xed in 75% ethanol for 24 hours at -20 °C, and the cells were then stained with DNA staining solution (LiankeBio, Hangzhou, China) for 30 min to detect cell cycle distribution.
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