Hydrogel components, including 4-arm PEG acrylate (MW 10,000) and HS-PEG-SH (MW 3,400), were obtained from Laysan Bio. For every 25 µL of 10 % (wt/v) cross-linked hydrogel, 1.6 mg of 4-arm PEG acrylate and 1.1 mg of HS-PEG-SH were dissolved in pH 7.4 PBS (Invitrogen). It was briefly vortexed and centrifuged to ensure mixing and it was allowed to sit on bench for 10 min while the hydrogel components cross-linked through the reaction between the thiol and acrylate groups.
4 arm peg acrylate
Manufactured by Thermo Fisher Scientific
4-arm PEG acrylate is a polyethylene glycol-based cross-linking agent. It contains four acrylate functional groups that can be used to form hydrogels through photo-initiated or chemical cross-linking reactions.
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Lab products found in correlation
2 protocols using 4 arm peg acrylate
1
PEG-based Hydrogel Crosslinking Protocol
2
PEG-based Hydrogel Crosslinking Protocol
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Hydrogel components, including 4-arm PEG acrylate (MW 10,000) and HS-PEG-SH (MW 3,400), were obtained from Laysan Bio. For every 25 µL of 10 % (wt/v) cross-linked hydrogel, 1.6 mg of 4-arm PEG acrylate and 1.1 mg of HS-PEG-SH were dissolved in pH 7.4 PBS (Invitrogen). It was briefly vortexed and centrifuged to ensure mixing and it was allowed to sit on bench for 10 min while the hydrogel components cross-linked through the reaction between the thiol and acrylate groups.
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