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Human collagen 1 alpha 1 elisa kit

Manufactured by Novus Biologicals
Sourced in United States

The Human Collagen I alpha 1 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human collagen I alpha 1 in biological samples.

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2 protocols using human collagen 1 alpha 1 elisa kit

1

Collagen I Production in Hs68 Cells

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The Hs68 cells were seeded evenly in 6-well-plates (2 × 105 cells/well), washed twice with phosphate-buffered saline (PBS), replaced with serum-free medium, and treated with various heat-killed Lactobacillus strains at 10-fold (1 × 109 cells/well) and 20-fold (5 × 108 cells/well) dilutions for 24 h. The supernatant was collected for analysis using a Human Collagen I alpha 1 ELISA Kit (Novus Biologicals LCC, Centennial, CO, USA).
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2

Quantifying Collagen I and Hyaluronic Acid

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The supernatant was analyzed for type I collagen and hyaluronic acid (HA) content per the manufacturer’s instructions using a Human Collagen I Alpha 1 ELISA kit (Novus Biologicals, Centennial, CO, USA) or a Hyaluronan Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA).
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