To evaluate the ability of the TPPA vector to encapsulate siRNA, gel electrophoresis experiments were performed. TPPA/siRNA (0.5 μg siRNA) nanocomplexes were prepared at various w/w ratios before mixing with 6× Glycerol Gel Loading Buffer (Sangon Biotech). Then the samples were electrophoresed in a 1% (w/v) agarose gel containing 4S Green Plus Nucleic Acid Stain (Sangon Biotech) and in TAE running buffer at 120 V for 20 min.
Glycerol gel loading buffer
Manufactured by Sangon
Glycerol Gel Loading Buffer is a solution designed for use in gel electrophoresis. It is used to prepare samples for loading onto a gel, helping to increase the density of the sample and allowing it to sink into the gel wells. The buffer contains glycerol, which increases the sample's density, and tracking dyes, which allow the progress of electrophoresis to be monitored.
Automatically generated - may contain errors
2 protocols using glycerol gel loading buffer
1
Evaluating siRNA Encapsulation by TPPA
2
Optimized Protocols for Mycotoxin Detection
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All DNA sequences (shown in Table S1 ) were synthesized by Sangon Biotech. Co., Ltd. (Shanghai, China) and purified using high-performance liquid chromatography (HPLC). Tris (2-carboxyethyl), Tris(2-carboxyethyl) phosphine hydrochloride (TCEP), 6× Glycerol Gel Loading Buffer, and 10× TM buffer (80 mM MgSO4; pH 7.4) were also obtained from Sangon Biotech. Co., Ltd. (Shanghai, China). Chloroauric acid (HAuCl4), NaCl, MgCl2, Trisodium citrate, and H2O2 were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Hemin, 2-methylimidazole, 3,3′,5,5′-tetramethylbenzidine (TMB), Tween 20, and Tris-HCl buffer (pH 7.4) were provided by Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). The AFB1 ELISA kit was bought from BEOSEN Food Safety Technology Co., Ltd. (Wuxi, China). Aflatoxin B1 (AFB1), deoxynivalenol (DON), zearalenone (ZEN), fumonisin B1 (FB1), aflatoxin B2 (AFB2), and ochratoxin (OTA) were obtained from Pribolab Co., Ltd. (Qingdao, China). Ultrapure water (18.2 MΩ·cm) was prepared through the Millipore water purification system.
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