UtVSMCs from pregnant rats (GD16) were isolated from the main uterine arteries using an enzymatic digestion method (24 (link)). Cell pellets were resuspended in DMEM (with 10% FBS and 100 U/mL penicillin, 100 µg/mL streptomycin), and cell solutions were distributed in culture dishes and kept in a humidified incubator at 37°C (5% CO2). Cell culture media was replaced every 48 h. Purity of utVSMCs was tested by immunocytochemistry for positive staining of VSMC marker smooth muscle actin (anti-α-smooth muscle actin, 1:400, Sigma Cat. No. A2547; secondary: anti-mouse Alexa 488, 1:500, Abcam, Cat. No. ab150113). Anti platelet endothelial cell adhesion molecule 1 (PECAM 1; CD31, 1:3,200, Cell Signaling, Cat. No. 3528S) was used as a negative control. Cultured utVSMCs were used at passage 2 to avoid genotypic and phenotypic drifts.
Anti mouse alexa488
Manufactured by Abcam
Anti-Mouse-Alexa488 is a secondary antibody that binds to mouse primary antibodies. It is conjugated to the Alexa Fluor 488 fluorescent dye, which emits green fluorescence when excited with the appropriate wavelength of light. This product can be used for immunofluorescence and flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti ki67, by Abcam (2 mentions)
Igg1 isotype control, by BD (2 mentions)
Hrp conjugated anti mouse and anti rabbit igg, by Jackson ImmunoResearch (2 mentions)
L mimosine, by Merck Group (2 mentions)
Akt inhibitor 8, by Merck Group (2 mentions)
Click it edu alexa fluor 488 imaging kit, by Thermo Fisher Scientific (2 mentions)
Function blocking anti human icam 1 15.2, by Bio-Rad (2 mentions)
Anti cd11b cd18 mab cbrm1 29, by Cell Signaling Technology (2 mentions)
Rabbit anti akt and anti β catenin phospho forms, by Cell Signaling Technology (2 mentions)
Anti human mhc class 1 1 b 548, by Abcam (2 mentions)
Dmem including media supplements, by Merck Group (2 mentions)
Cd31 pecam 1, by Cell Signaling Technology (1 mentions)
Anti α smooth muscle actin, by Merck Group (1 mentions)
3 protocols using anti mouse alexa488
1
Isolation and Characterization of Uterine Vascular Smooth Muscle Cells
2
Functional Blocking of Leukocyte Adhesion
3
Functional Blocking of Leukocyte Adhesion
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Function blocking anti-human ICAM-1 (15.2) from Serotec (Raleigh, NC), anti-mouse ICAM-1mAb (YN1/1.7.4), anti-Ly-6G conjugated to Alexa 488 and anti-mouse MHC Class-I (W6/32) from eBioscience (San Diego, CA), anti-CD11b/CD18 mAb (CBRM1/29) purified in-house as previously described,59 (link) Rabbit anti-Akt and anti-β-catenin phospho forms from Cell Signaling (Danvers, MA), anti-Mouse-Alexa488 (Carlsbad, CA), anti-human MHC Class-I (1.B.548) and Rabbit anti-Ki67 from Abcam (Cambridge, MA), Isotype control IgG1 from BD Biosciences (San Jose, CA), HRP-conjugated anti-mouse and anti-rabbit IgGs from Jackson Immunoresearch (West Grove, PA). Click-iT® EdU Alexa Fluor® 488 Imaging Kit Molecular Probes (Eugene, Oregon), Akt inhibitor VIII (Billerica, Massachusetts, EMD Millipore), ABTS (2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid), L-mimosine, HBSS with Ca2+ and Mg2+ (HBSS+) and HBSS without Ca2+ and Mg2+ (HBSS-), DMEM including media supplements as well as chemotactic peptide fMLF, mouse IFNγ and TNFα from Sigma (St Louis, MO).
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