Colorimetric triglyceride quantification assay

Manufactured by Merck Group

The Colorimetric triglyceride quantification assay is a laboratory equipment product designed to measure the concentration of triglycerides in a sample. It uses a colorimetric method to quantify the triglyceride levels.

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Lab products found in correlation

Total cholesterol assay kit, by Cell Biolabs (1 mentions)

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Colorimetric assay (65 citations)

2 protocols using colorimetric triglyceride quantification assay

Triglyceride Quantification in Cell Lysates

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Cell pellets were lysed in radioimmune precipitation assay (RIPA) buffer and protein concentration was determined with the bicinchoninic acid assay (Pierce) as the above western blot analysis. Total triglycerides were quantified using a colorimetric triglyceride quantification assay (Sigma) (n = 15 samples/treatment group). Triglyceride content was determined relative to protein content.

Nyunt T., Britton M., Wanichthanarak K., Budamagunta M., Voss J.C., Wilson D.W., Rutledge J.C, & Aung H.H. (2019). Mitochondrial oxidative stress-induced transcript variants of ATF3 mediate lipotoxic brain microvascular injury. Free radical biology & medicine, 143, 25-46.

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Quantification of Lipid Markers

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Cells were lysed after treatment with lysis buffer (50 mM Tris, 150 mM Na Cl, 1% Nonidet P-40 Substitute, 0.25% sodium deoxycholate, 0.1% sodium dodecyl sulfate, pH 7.4). Total triglycerides were quantified using a colorimetric triglyceride quantification assay (Sigma). Total cholesterol was quantified using a colorimetric Total Cholesterol Assay Kit (Cell Biolabs Inc.). The cell lysate was also assayed for protein content by a BCA protein assay kit (Pierce). Triglyceride and cholesterol content were determined relative to protein content to control for any differences in cell numbers.

Norman J.E., Aung H.H., Wilson D.W, & Rutledge J.C. (2018). Inhibition of perilipin 2 expression reduces pro-inflammatory gene expression and increases lipid droplet size. Food & function, 9(12), 6245-6256.

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