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Formvar carbon coated cu grids

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Formvar/carbon-coated Cu grids are electron microscopy specimen support grids made of copper. They feature a thin polymer film (Formvar) coated with a layer of amorphous carbon. This combination provides a stable and conductive support for samples during electron microscope analysis.

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Lab products found in correlation

4k ccd camera, by Ametek (2 mentions) Sphera transmission electron microscope, by Ametek (2 mentions) Sigma 500 scanning electron microscope, by Zeiss (1 mentions)

4 protocols using formvar carbon coated cu grids

1

Characterization of BMC Cages

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A 4-μL droplet of BMC cages (either self-assembled or from dissolved crystals) was deposited onto formvar/carbon-coated Cu grids (Ted Pella, Inc.) (pretreated by negative-mode glow discharge up to 15 min prior) and allowed to bind for 5 min. The grids were then washed with 50 μL of MilliQ water, blotted using Whatman filter paper and stained using 2% uranyl acetate solution in water and blotted again. Grids were imaged using a FEI Sphera transmission electron microscope operating at 200 keV, equipped with a LaB6 filament and a Gatan 4K CCD camera. Micrographs were collected using objective-lens underfocus settings ranging from 250 nm to 2 μm and analyzed using Fiji (http://fiji.sc/Fiji).
Golub E., Subramanian R.H., Esselborn J., Alberstein R.G., Bailey J.B., Chiong J.A., Yan X., Booth T., Baker T.S, & Tezcan F.A. (2020). Constructing Protein Polyhedra via Orthogonal Chemical Interactions. Nature, 578(7793), 172-176.
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2

Characterization of BMC Cages

Check if the same lab product or an alternative is used in the 5 most similar protocols
A 4-μL droplet of BMC cages (either self-assembled or from dissolved crystals) was deposited onto formvar/carbon-coated Cu grids (Ted Pella, Inc.) (pretreated by negative-mode glow discharge up to 15 min prior) and allowed to bind for 5 min. The grids were then washed with 50 μL of MilliQ water, blotted using Whatman filter paper and stained using 2% uranyl acetate solution in water and blotted again. Grids were imaged using a FEI Sphera transmission electron microscope operating at 200 keV, equipped with a LaB6 filament and a Gatan 4K CCD camera. Micrographs were collected using objective-lens underfocus settings ranging from 250 nm to 2 μm and analyzed using Fiji (http://fiji.sc/Fiji).
Golub E., Subramanian R.H., Esselborn J., Alberstein R.G., Bailey J.B., Chiong J.A., Yan X., Booth T., Baker T.S, & Tezcan F.A. (2020). Constructing Protein Polyhedra via Orthogonal Chemical Interactions. Nature, 578(7793), 172-176.
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3

SEM Imaging of Ferritin Crystals

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Native ferritin crystal and crystal-hydrogel hybrid samples were deposited onto glow-discharged, Formvar/carbon-coated Cu grids (Ted Pella Inc.). Each grid was blotted with filter paper to remove excess liquid. Grids were mounted onto a STEM 12x v2 sample holder and imaged using a Sigma 500 scanning electron microscope (Zeiss) at an accelerating voltage of 1 kV using a 30-μm aperture.
Zhang L., Bailey J.B., Subramanian R.H., Groisman A, & Tezcan F.A. (2018). Hyperexpandable, self-healing macromolecular crystals with integrated polymer networks. Nature, 557(7703), 86-91.
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4

Negative Staining for TEM Imaging

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A 3-μL suspension of crystals was pipetted onto formvar/carbon-coated Cu grids (Ted Pella, Inc.) that had been glow discharged for 45-60 s. Samples were incubated for 5 min, washed with 50 μL of MilliQ water and blotted with filter paper. A 3-μL drop of 1% uranyl acetate in water was pipetted onto the grid and incubated for 30-60 s and blotted dry with filter paper. Grids were imaged using a FEI Sphera transmission electron microscope operating at 200 keV, equipped with a LaB6 filament and a Gatan 4K charged-coupled device (CCD). Micrographs were collected using objective-lens underfocus settings ranging from 250 nm to 2 μm and analyzed using Fiji (http://fiji.sc/Fiji).
Subramanian R.H., Suzuki Y., Tallorin L., Sahu S., Thompson M., Gianneschi N.C., Burkart M.D, & Tezcan F.A. (2020). Enzyme-Directed Functionalization of Designed, Two-Dimensional Protein Lattices. Biochemistry, 60(13), 1050-1062.
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