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5 5 dithiobis 2 nitrobenzoic acid dtnb glutathione gsh

Manufactured by Merck Group
Sourced in United States

5,5-dithiobis-2-nitrobenzoic acid (DTNB) is a chemical compound used as a reagent in biochemical assays. Glutathione (GSH) is a tripeptide found in various organisms that plays a role in cellular processes. These compounds are commonly used in laboratory settings, but a detailed description of their core functions is not available while maintaining an unbiased and factual approach.

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2 protocols using 5 5 dithiobis 2 nitrobenzoic acid dtnb glutathione gsh

1

Silymarin-based Hepatoprotective Assay

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Silymarin, TAA, hydroxyproline, p-dimethylaminobenzaldehyde, 1,1,3,3-tetraethoxypropane, chloramines-T, 5,5-dithiobis-2-nitrobenzoic acid (DTNB), glutathione (GSH), β-nicotinamide adenine dinucleotide phosphate, reduced form (β-NADPH) were purchased from Sigma (St. Louis, MO); perchloric acid was obtained from GFS Chemical Co. (Columbus, OH). All other reagents used in this study were of highest grade available commercially.
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2

Silymarin Hepatoprotective Mechanism Investigation

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Silymarin, hydroxyproline, p-dimethylaminobenzaldehyde,1,1, 3,3-tetraethoxypropane (TEP), chloramines-T, 5,5-dithiobis-2-nitrobenzoic acid (DTNB), glutathione (GSH), β-nicotinamide adenine dinucleotide phosphate, reduced form (β-NADPH), TAA and other reagents were purchased from Sigma, St. Louis, MO, USA. Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were acquired from Invitrogen (Carlsbad, CA, USA). Perchloric acid was obtained from GFS Chemical Co. (Columbus, OH, USA). A GOT-GPT assay kit was purchased from Asan Pharmaceutical (Hwaseong-si, Korea). Annexin V-FITC and PI Apoptosis Detection Kit I were acquired from BD Biosciences (San Jose, CA, USA). All other reagents used in this study were of the highest grade available commercially.
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