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47 protocols using aeroset

1

Metabolic Biomarker Assessment Protocol

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Plasma triglycer ide, total cholesterol, LDL, HDL concentrations, and fasting glucose were mea sured using an automated chemistry an alyzer (Aeroset; Abbott, Holliston, MN, USA) with commercial kits (Abbott). C-reactive protein (CRP) was measured using an autoanalyz er (Aeroset; Abbott) with a spectrophoto metric commercial kit (Scil Diagnostics GmbH, Viernheim, Germany). Serum GGT activities were measured with the en zymatic calorimetric test (Roche/Hitachi analyzer, Mannheim, Germany) and the normal range of GGT activity was iden tifi ed as 7-49 U/l.
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Serum Biomarkers for Cardiac Evaluation

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The serum levels of lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and cardiac troponin I (cTnI) were determined using an automatic biochemical analyzer (Abbott Aeroset TM, USA).
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3

Biochemical Analysis of Mice Serum

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An Abbott AerosetTM biochemical analyzer (Abbott Laboratories, Abbott Park, IL, USA) was utilized to measure the serum levels of TC, TG, HDLC, LDLC and calcium in the mice.
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4

Serum Biomarker Analysis Protocol

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The blood samples were centrifuged at 3000 rpm for 10 min to separate serum for biochemical analysis. Serum albumin, glucose, cholesterol, triglyceride, HDL-ch and LDL-ch were measured using an Abbott Aeroset automated instrument analyzer (Abbott Diagnostics, Abbott Park, Illinois, USA). LDL-ch and HDL-ch were detected as cholesterol content in serum lipoprotein molecules and are regarded here as representative of serum LDL and HDL levels. Serum concentrations of estradiol and testosterone were also determined using an Abbott AXSYM System automated immunoassay analyzer (Abbott Diagnostics, Abbott Park, Illinois, USA).
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5

Metabolic and Cytokine Profiling

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TC, HDL and TG concentrations were measured at the Clinical Biochemistry Service, Cardiff University, on an Aeroset automated analyzer (Abbott Diagnostics, Berkshire, UK). Plasma VLDL/LDL concentrations were measured using the VLDL/LDL cholesterol assay kit (ABCAM, Cambridge, UK) in accordance with the manufacturer’s instructions. Plasma cytokine concentrations were measured by the Central Biotechnology Service (Cardiff University, UK) using the VPLEX pro-inflammatory panel 1 mouse kit (Meso Scale Discovery, Maryland, USA).
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6

Comprehensive Hormonal and Metabolic Profiling

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Testosterone was measured by liquid chromatography–tandem mass spectrometry using Perkin Elmer’s CHS Steroid MS kit. Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were analyzed on the Architect i2000 platform (Abbott) by the chemiluminescent microparticle immunoassay method using the corresponding kits. The working ranges were 0.05 to 150 IU/L and 0.07 to 250 IU/L, respectively. Plasma lipids and triglycerides were measured using an automated commercially available system (Aeroset, Abbott Diagnostics, Abbott Park Laboratories). The coefficient of variation was <5%. Glycated hemoglobin A1c (HbA1c) was measured by commercially available high-performance liquid chromatography. Hepatic enzymes were determined on a Cobas INTEGRA (Roche). Prostate-specific antigen (PSA) was measured on ADVIA Centaur/XPT. Normative values for these assays are presented in Figure legends.
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Comprehensive Blood Biomarker Profiling

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A full blood count was measured on an ABX Pentra X120 haematology blood analyser (Horiba, Northampton, UK). Serum cholesterol, high-density lipoprotein cholesterol and triglycerides were assessed using an Aeroset automated analyser (Abbott Diagnostics, Berkshire, UK). Low-density lipoprotein cholesterol was calculated using Friedewald's formula. C-reactive protein was assayed by nephelometry (BN-II system, Milton Keynes, UK). All biochemical measurements were performed by the Department of Medical Biochemistry,UHW.
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8

Comprehensive Biochemical Profiling

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Routine biochemistry was undertaken by the hospital biochemistry laboratory on the Aeroset (ALT, AST, HDL, triglyceride) or Architect (insulin) clinical chemistry analyser platforms (Abbott Diagnostics, Illinois, USA). Insulin concentrations were determined using a one-step chemiluminescent immunoassay. Cytokine analysis was performed by Aushon Multiplex Immunoassay Analysis (Aushon Biosystems, Billerica, USA).
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9

Renal Fibrosis Blood Biomarker Study

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A total of 10 patients with renal fibrosis were enrolled from The First Affiliated Hospital of Wenzhou Medical College, China. Percutaneous renal biopsy samples were obtained from 10 patients after a renal fibrosis diagnosis was confirmed, and renal biopsies from 10 patients without fibrotic lesions were used as controls. Patients were excluded if any of the following was present: polycystic kidney disease, pregnancy, human immunodeficiency virus, renal cancer, or recent immunosuppressive therapy. All patients had signed the informed consent forms. We collected the peripheral blood from each of the patients before and after taking Nephropathy 1st and further separated the serum to detect the creatinine (CREA) and urine protein by Abbott Aeroset (Abbott, Abbott Park, IL).
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10

Biochemical Markers of Liver Injury and Fibrosis in Rats

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Blood samples were acquired from rats at each time point, placed at room temperature for 30 minutes, and then centrifuged for 15 minutes at 3000 rpm (Sorvall® Biofuge Stratos, Thermo, Germany), and the serum was collected. Then, albumin (ALB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (TBIL), direct bilirubin (DBIL), gamma-glutamyl transpeptidase (γ-GT) concentrations were assessed using an automated biochemical analyzer (Abbott Aeroset, Abbott Laboratories, Chicago, IL, USA). The levels of hepatic fibrogenesis indicators, such as laminin (LN) and hyaluronan (HA), were measured using commercial enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems Inc., Minneapolis, USA and Abnova Corporation, Taiwan) according to the manufacturer's instructions.
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