Fmoc-GGGK-CONH2 was dissolved in DMSO to a final concentration of 100 mM, then combined with 1.5 equivalents of LC-SMCC (Thermo-Fisher) and 2 equivalents of DIPEA (Sigma) in DMSO. The reaction was incubated for 1 hr at room temperature, then combined with 1.5 equivalents of coenzyme A trilithium hydrate (Sigma) in DMSO to a final peptide concentration of 25 mM and mixed at room temperature overnight. The Fmoc protecting group was removed with 20% vol/vol piperidine and incubation for 20 minutes. The reaction was quenched by the addition of 1 equivalent of TFA, and the product was purified on a preparative Kromasil 100–5-C18 column (21.2×250 mm, Peeke Scientific) by reverse phase HPLC (flow rate: 9.5 mL/min; gradient: 10% to 70% acetonitrile with 0.1% TFA in 0.1% aqueous TFA gradient over 30 minutes; retention time: 17.1 minutes). ESI-MS: [M-H]−m/z = 1300.1 (observed); calculated for C45H72N14O23P3S− = 1301.4. The concentration of GGGK-CoA peptide was determined from the measured A259 using the known molar extinction coefficient of coenzyme A 53 , 15,000 M−1 cm−1.
Coenzyme a trilithium hydrate
Manufactured by Merck Group
Coenzyme A trilithium hydrate is a chemical compound that serves as a cofactor in various enzymatic reactions in biological systems. It is a salt form of coenzyme A, which is an essential molecule involved in the metabolism of carbohydrates, fats, and proteins. The trilithium hydrate form provides a stable and water-soluble variant of coenzyme A for use in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using coenzyme a trilithium hydrate
1
Synthesis and Purification of GGGK-CoA
2
Synthesis and Purification of GGGK-CoA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fmoc-GGGK-CONH2 was dissolved in DMSO to a final concentration of 100 mM, then combined with 1.5 equivalents of LC-SMCC (Thermo-Fisher) and 2 equivalents of DIPEA (Sigma) in DMSO. The reaction was incubated for 1 hr at room temperature, then combined with 1.5 equivalents of coenzyme A trilithium hydrate (Sigma) in DMSO to a final peptide concentration of 25 mM and mixed at room temperature overnight. The Fmoc protecting group was removed with 20% vol/vol piperidine and incubation for 20 minutes. The reaction was quenched by the addition of 1 equivalent of TFA, and the product was purified on a preparative Kromasil 100–5-C18 column (21.2×250 mm, Peeke Scientific) by reverse phase HPLC (flow rate: 9.5 mL/min; gradient: 10% to 70% acetonitrile with 0.1% TFA in 0.1% aqueous TFA gradient over 30 minutes; retention time: 17.1 minutes). ESI-MS: [M-H]−m/z = 1300.1 (observed); calculated for C45H72N14O23P3S− = 1301.4. The concentration of GGGK-CoA peptide was determined from the measured A259 using the known molar extinction coefficient of coenzyme A 53 , 15,000 M−1 cm−1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!