Anti cd3 pacific blue and anti cd4 percp antibody

Manufactured by BioLegend

Sourced in United States

The Anti-CD3-Pacific Blue and Anti-CD4-PerCP antibodies are fluorochrome-conjugated monoclonal antibodies designed for the identification and enumeration of T lymphocyte subsets in flow cytometry applications.

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Lab products found in correlation

Fortessa, by BD (1 mentions)

Spelling variants of this product

Anti-CD3 antibody (77 citations) CD3-PerCP (50 citations) CD4-PerCP (30 citations) Anti-CD4 Pacific Blue (28 citations) Anti-CD3-PerCP (28 citations) CD3-Pacific Blue (26 citations) CD4-Pacific Blue (24 citations) Anti-CD4-PerCP (21 citations) Anti-CD3-Pacific Blue (17 citations) Anti-CD4 antibody (12 citations)

2 protocols using anti cd3 pacific blue and anti cd4 percp antibody

HLA Class II Tetramer Production and Analysis

Cited 1 time

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HLA class II tetramers were produced as previously described[41] (link). Briefly, recombinant HLA-DR α- and β-chains were folded in the presence of a C-terminally hexahistidine(H₆)-tagged version of the peptide in question. The peptide-HLA class II complexes were subsequently purified on a Ni²⁺ charged iminodiacetic acid column. The resulting monomers were tetramerized with PE- or APC-conjugated Streptavidin as described for the HLA class I tetramer above. In vitro cultured PBMCs were incubated with PE- and APC-conjugated HLA class II tetramers for 1 h at 37°C, 5% CO₂. The cells were washed and subsequently stained with anti-CD3-Pacific blue and anti-CD4-PerCP antibody (Biolegend, San Diego, USA) for 30 min. All tetramer-stained cells were analyzed by flow cytometry on LSRII (BD Biosciences).

Braendstrup P., Mortensen B.K., Justesen S., Østerby T., Rasmussen M., Hansen A.M., Christiansen C.B., Hansen M.B., Nielsen M., Vindeløv L., Buus S, & Stryhn A. (2014). Identification and HLA-Tetramer-Validation of Human CD4+ and CD8+ T Cell Responses against HCMV Proteins IE1 and IE2. PLoS ONE, 9(4), e94892.

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Characterizing Antigen-Specific T Cell Responses

Cited 2 times

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HLA class II tetramers were produced as previously described. Briefly, recombinant α- and β-chains (HLA-DRA1 and DRB5*01:01) were folded in the presence of either the wild-type IE1_211-225 peptide, P3 variant, or P7 variant of the wild-type peptide. The resulting monomers were tetramerized with PE- or APC-conjugated streptavidin.
PBMC's from a donor previously determined to recognize the DRB5*01:01 restricted CMV IE1_211–225 epitope (Braendstrup et al. 2014 (link)) was expanded on this epitope. The cells were double-stained with PE-labeled wild-type IE1_211–225 -DRB5*01:01 tetramer and APC-labeled mutant peptide-DRB5*01:01 tetramer as previously described (Braendstrup et al. 2013 (link)). The cells were washed and subsequently stained with anti-CD3-Pacific blue and anti-CD4-PerCP antibody (Biolegend, San Diego, USA) for 30 min. The stained cells were analyzed by flow cytometry on a Fortessa (BD Biosciences).

Andreatta M., Karosiene E., Rasmussen M., Stryhn A., Buus S, & Nielsen M. (2015). Accurate pan-specific prediction of peptide-MHC class II binding affinity with improved binding core identification. Immunogenetics, 67(0), 641-650.

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