Human primary chondrocytes subjected to different treatments were collected using trypsin without EDTA (Thermo Fisher Scientific) and washed twice with PBS. For the apoptosis assay, the cells were stained using an Annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) Apoptosis Kit (BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer’s instructions and then detected using a BD FACS flow cytometer (BD Biosciences). For the cell cycle assay, the cells were fixed overnight in absolute ethanol, washed twice, and incubated with PI/RNase staining buffer (BD Biosciences, Franklin Lakes, NJ, USA), and the specimens were then detected using a BD FACS flow cytometer (BD Biosciences).
Fitc pi apoptosis kit
Manufactured by BD
Sourced in United States
The FITC/PI Apoptosis Kit is a laboratory reagent used to detect and quantify apoptosis, a form of programmed cell death, in cell samples. The kit utilizes fluorescent dyes, FITC (Fluorescein Isothiocyanate) and PI (Propidium Iodide), to label and differentiate between viable, apoptotic, and necrotic cells.
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2 protocols using fitc pi apoptosis kit
1
Chondrocyte Apoptosis and Cell Cycle Assays
2
Quantifying Cellular Apoptosis by Flow Cytometry
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Cell apoptosis was quantified using propidium iodide (PI) and Annexin V staining. After Dex and I/R treatments, Neuro-2a cells were subjected to flow cytometric analysis to detect apoptosis using the Annexin V-fluorescein isothiocyanate (FITC)/PI Apoptosis Kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s protocol. Briefly, after washing with PBS and the binding buffer for one time each, cells were stained with Annexin V-FITC/PI for 20 min at room temperature in dark. After washing with the binding buffer once, the labeled cells were detected immediately by a flow cytometer (Beckman Coulter, Franklin Lakes, NJ, USA). The percentage of apoptotic cells was defined as the % of Annexin V-positive cells (including both PI-negative and PI-positive populations) among the whole cells.
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