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Ab130769

Manufactured by Abcam
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Ab130769 is a laboratory reagent. It is a recombinant protein that can be used in various biochemical and immunological applications. The core function of this product is to serve as a research tool for scientific investigations.

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Lab products found in correlation

Ab134936, by Abcam (2 mentions) Ab8245, by Merck Group (2 mentions) Ab16478, by Abcam (2 mentions) Casein blocking buffer, by Merck Group (2 mentions) C6219, by Merck Group (2 mentions) Alexa fluor 680, by Thermo Fisher Scientific (2 mentions) Irdye 800, by Rockland Immunochemicals (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions) Nitrocellulose membrane, by Bio-Rad (2 mentions)

2 protocols using ab130769

1

Western Blot Analysis of Alzheimer's Biomarkers

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Brain homogenates (frontal cortex) in urea (50 μg) were mixed with Laemmli sample buffer and resolved by SDS-PAGE before an overnight wet transfer to nitrocellulose membranes (BioRad) as previously reported (Seyfried et al., 2017 (link)). Membranes were blocked with casein blocking buffer (Sigma B6429) and probed with primary polyclonal antibodies for Anti-PLCD1 antibody (Abcam #ab134936), anti-HEPACAM (Abcam #ab130769), anti-PADI2 antibody (Abcam #ab16478), anti-GAPDH antibody (Abcam #ab8245), and anti-Connexin-43 (Sigma #C6219) overnight at 4°C. Membranes were incubated with secondary antibodies conjugated to Alexa Fluor 680 (Invitrogen) or IRDye800 (Rockland) fluorophores for one hour at room temperature. Images were captured using an Odyssey Infrared Imaging System (Li-Cor Biosciences). Protein densitometry for relative quantification was performed using ImageJ open source software. For GJA1, the higher molecular weight band reflective of ubiquitination (Abreha et al., 2018 (link)) was analyzed separately from the lower molecular weight band. Densitometry was compared between AD and control using the Student’s t test (Student, 1908 ).
Swarup V., Chang T.S., Duong D.M., Dammer E.B., Dai J., Lah J.J., Johnson E.C., Seyfried N.T., Levey A.I, & Geschwind D.H. (2020). Identification of Conserved Proteomic Networks in Neurodegenerative Dementia. Cell reports, 31(12), 107807.
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2

Western Blot Analysis of Alzheimer's Biomarkers

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Brain homogenates (frontal cortex) in urea (50 μg) were mixed with Laemmli sample buffer and resolved by SDS-PAGE before an overnight wet transfer to nitrocellulose membranes (BioRad) as previously reported (Seyfried et al., 2017 (link)). Membranes were blocked with casein blocking buffer (Sigma B6429) and probed with primary polyclonal antibodies for Anti-PLCD1 antibody (Abcam #ab134936), anti-HEPACAM (Abcam #ab130769), anti-PADI2 antibody (Abcam #ab16478), anti-GAPDH antibody (Abcam #ab8245), and anti-Connexin-43 (Sigma #C6219) overnight at 4°C. Membranes were incubated with secondary antibodies conjugated to Alexa Fluor 680 (Invitrogen) or IRDye800 (Rockland) fluorophores for one hour at room temperature. Images were captured using an Odyssey Infrared Imaging System (Li-Cor Biosciences). Protein densitometry for relative quantification was performed using ImageJ open source software. For GJA1, the higher molecular weight band reflective of ubiquitination (Abreha et al., 2018 (link)) was analyzed separately from the lower molecular weight band. Densitometry was compared between AD and control using the Student’s t test (Student, 1908 ).
Swarup V., Chang T.S., Duong D.M., Dammer E.B., Dai J., Lah J.J., Johnson E.C., Seyfried N.T., Levey A.I, & Geschwind D.H. (2020). Identification of Conserved Proteomic Networks in Neurodegenerative Dementia. Cell reports, 31(12), 107807.
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