Coconut cake was obtained from South Coconut Food Processing Co., China. Flavourzyme (5 × 104 U g−1), alcalase (1 × 105 U g−1), pepsin (1 × 105 U g−1) and trypsin (1 × 105 U g−1) were purchased from Shanghai Biotech. Co., Ltd. (China). ACE (from rabbit lung) and N-Hippuryl-His-Leu hydrate (HHL) were purchased from Sigma Co., USA. The human endothelial cell line (EA.hy926) was from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Sijiqing Biological Co., China. Human endothelin-1 (ET-1) Elisa kit was purchased from Ruiqi Biological Co. Ltd., China. GSH (glutathione), ferrozine and 2-Deoxy-d -ribose were purchased from Yeyuan Biotech. Co., Ltd., Shanghai, China. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and other chemicals and reagents were of analytical grade. Three isolated peptides (KAQYPYV, KIIIYN and KILIYG) were synthesized using the method of the solid phase procedure described by Zhang et al.25
Ace from rabbit lung
Manufactured by Merck Group
Sourced in United States
ACE from rabbit lung is a laboratory product used for research purposes. It functions as an angiotensin-converting enzyme, which plays a role in regulating blood pressure. This product is intended for use in scientific investigations and experiments, and its specific applications may vary depending on the research context.
Automatically generated - may contain errors
Lab products found in correlation
Millex gv, by Merck Group (4 mentions)
Millex lg, by Merck Group (4 mentions)
Trifluoroacetic acid tfa, by Fujifilm (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Hippuryl histidyl leucine, by Merck Group (3 mentions)
N hippuryl his leu hydrate hhl, by Merck Group (2 mentions)
Synthetic peptides, by Medical & Biological Laboratories (2 mentions)
Pepsin, by Merck Group (2 mentions)
Hippuryl l histidyl l leucine hhl, by Merck Group (2 mentions)
Ea hy926 cells, by National Collection of Authenticated Cell Cultures (2 mentions)
Ea hy926, by National Collection of Authenticated Cell Cultures (1 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt, by Merck Group (1 mentions)
N hippuryl his leu hydrate powder, by Merck Group (1 mentions)
Folin ciocalteu phenol reagent, by Merck Group (1 mentions)
α glucosidase from saccharomyces cerevisiae, by Merck Group (1 mentions)
2 4 6 tri 2 pyridyl s triazine, by Merck Group (1 mentions)
Lipase from porcine pancreas, by Merck Group (1 mentions)
Organic solvents, by Merck Group (1 mentions)
Hippuric acid, by Merck Group (1 mentions)
N hippuryl l histidyl l leucine hhl, by Merck Group (1 mentions)
23 protocols using ace from rabbit lung
1
Antioxidant and ACE Inhibitory Peptides from Coconut Cake
2
Purification and Characterization of Uppurui Nori ACE
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Uppurui Nori, P. pseudolinearis, was collected from the coast of Hakodate, Japan. The sample was stored at –30 °C until use. ACE from rabbit lung was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Millex-GV (pore size: 0.22 µm) and Millex-LG (pore size: 0.20 µm) were purchased from MERCK MILLIPORE Ltd. (Billerica, MA, USA). Synthetic peptides (purity: ≥95%) were purchased from Medical Biological Laboratories Co. (Nagoya, Japan). Hyppuryl-L-histidyl-L-leucine (Hip-His-Leu), thermolysin (EC 3.4.24.27) from Bacillus thermoproteolyticus, trifluoroacetic acid (TFA), and all other regents were purchased from Fujifilm Wako Pure Chemical (Osaka, Japan).
3
Antioxidant and Enzyme Inhibition Assays
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4‐Nitrophenyl Laurate were purchased from TCL (Shanghai, China). 2,2‐Diphenyl‐1‐picrylhydrazyl (DPPH), 2,2′‐azinobis (3‐ethyl‐benzothiazoline‐6‐sulfonic acid) diammonium salt (ABTS), 2,4,6‐tri (2‐pyridyl)‐s‐triazine (TPTZ), N‐Hippuryl‐His‐Leu hydrate powder (HHL), the Folin–Ciocalteu phenol reagent, lipase from porcine pancreas, ACE (from rabbit lung), and α‐Glucosidase from Saccharomyces cerevisiae were purchased from Sigma Chemical Co (St. Louis, MO, USA). 6‐Hydroxy‐2,5,7,8‐tetramethylchromane‐2‐carboxylic acid (Trolox), 4‐N‐trophenyl‐α‐D‐glucopyranoside (PNPG), Standards of gallic acid and quertein, Triton X‐100, and Tris‐HCL were purchased from Aladdin (Shanghai, China). Other reagents were all of analytical grade.
4
Spectrophotometric ACE Inhibition Assay
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ACE-inhibitory activity (IC50) was assayed by the spectrophotometric method with the use of hippury-l -histydyl-l -leucine (HHL) (Sigma, H4884) as substrate (Miguel et al. 2007 (link)). HHL (5 mmol/L in 100 mmol/L potassium phosphate containing 300 mmol/L sodium chloride, pH 8.3), enzyme (ACE from rabbit lung, Sigma, A6778) and peptide solutions were incubated at 37 °C for 30 min. The reaction was stopped with the addition of 1 mol/L HCl. Conversion of HHL to hippurate and l -histidyl-l -leucine was quantified spectrophotometrically at λ 228 nm. The IC50 was defined as the concentration of inhibitor required to inhibit 50 % of the ACE activity.
5
Antioxidant and ACE Inhibition Assays
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All chemicals, reagents and organic solvents of the highest grade available were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated. DPPH, ACE from rabbit lung, N-Hippuryl-l -histidyl-l -leucine (HHL) and Hippuric acid were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Deionized water was prepared by an Arium 611 VF system from Sartorius (Göttingen, Germany). Solid Phase Extraction (SPE) C18 cartridges were Bond Elut (Varian, Palo Alto, CA, USA).
Deionized water was prepared by an Arium 611 VF system from Sartorius (Göttingen, Germany). Solid Phase Extraction (SPE) C18 cartridges were Bond Elut (Varian, Palo Alto, CA, USA).
6
Walnut Meal-Based ACE Inhibition and Biomarker Analysis
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The Grain, Oil and Protein Engineering Laboratory of Shaanxi Normal University (Xi’an, China) provided the defatted walnut meal. ACE (from rabbit lung) and hippuryl-histidyl-leucine (HHL) were purchased from Sigma Co., St. Louis, MO, USA. Pepsin (3 × 103 U/g) was obtained from Solarbio Science & Technology, Co., Ltd. (Beijing, China). High-performance liquid chromatography grade acetonitrile and trifluoroacetic acid were purchased from Fischer Scientific Co. (Waltham, MA, USA). The enzyme-linked immunosorbent assay kit for the detection of ACE, angiotensinogen (AGT), aldosterone (ALD), and Endothelin-1(ET-1) was purchased from Jiangsu Jingmei Biotechnology Co., Ltd. (YanCheng, China). The enzyme-linked immunosorbent assay kit for the detection of Ang II was obtained from Shanghai Kexing Biotechnology Co., Ltd., (Shanghai, China). and the nitric oxide (NO) detection kit was a product of Nanjing Jiancheng Institute of Biological Engineering (Nanjing, China), and other chemical reagents used in the experiment were of analytical grade.
7
Enzymatic Inhibition of Angiotensin-Converting Enzyme
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Portuguese oysters (Crassostrea angulata) were purchased from Penghu Island, Taiwan. They were packed in a box with ice and sent to the laboratory by freight transport. Pepsin (from porcine gastric mucosa), bromelain (from pineapple stem), and papain (from papaya) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The angiotensin I converting enzyme (ACE) from rabbit lung (≥2 units/mg), N-(3-[2-furyl]-acryloyl)-phenylalanyl glycyl glycine (FAPGG), Dipeptidyl Peptidase IV (DPP-IV) from human recombinant (≥1 unit/mg), and Gly-Pro p-nitroanilide hydrochloride (≥99%) were also acquired from Sigma-Aldrich, USA. All chemical reagents used were of analytical grade.
8
Muscovy Duck Blood ACE Assay
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Muscovy ducks (Cairina moschata) blood was obtained from Anqing Yongqiang Agricultural Science and Technology Co., Ltd. (Anqing, China). Alkaline protease (S10154, 200 U/mg) was purchased from Yuanye Biotechnology (Shanghai, China). Bicinchoninic acid (BCA) protein assay kit (Biosharp, Shanghai, China) was employed for protein content determination. ACE from rabbit lung (0.1 U/mL) and hippuryl–L–histidyl–L–leucine (HHL) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile, formic acid and triethylamine were HPLC grade, while other reagents were of analytical grade.
9
Evaluation of Artichoke Flower Extract
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Mature artichoke flowers (C. scolymus L.) were collected from the Region of Murcia (Spain). Vacuum-packed A. diaperinus L. larvae were provided by Proti-Farma (Holland). ACE from rabbit lung, the ACE synthetic substrate, hippuryl-L-histidyl-L-leucine (HHL), and 1,1-diphenil-2-picrylhydrazyl) (DPPH) were purchased from Sigma Chemicals Co. (St. Louis, MO, USA).
10
Isolation and Characterization of Crude ACE
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Crude ACE was isolated according to the reported literature [44 (link)]. Hippuryl-histidyl-leucine (HHL; ≥99%) and ACE (from rabbit lung) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). 2-MeIM (≥99%), DA (≥99%) and HPLC-grade trifluoroacetic acid were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). DA-PEGx-DA (x = 2000, 5000 and 10,000) were purchased from Guangzhou Tansh-tech Co., Ltd. (Guangzhou, China). VPP and GF-6 were obtained from GL Biochem (Shanghai) Ltd. (Shanghai, China). HPLC-grade methanol and acetonitrile were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). All the other reagents were of analytical grade and purchased from XiLong Science (Guangdong, China).
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