Plumbagin was obtained from the LKT Laboratories (St. Paul, Minnesota, USA). Bradford solution was a product of Bio-Rad (Hercules, CA, USA). Eosin Y 1% aqueous solution and Mayer’s hematoxylin were from Bio Optica (Milan, Italy). Xylene and Permount® were bought from Fisher Scientific (Loughborough, UK). Alanine transaminase (ALT) and aspartate transaminase (AST), bovine serum albumin, β-nicotinamide adenine dinucleotide phosphate reduced form (NADPH), xanthine oxidase, nitroblue tetrazolium (NBT), 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB), SOD, CAT, GSH reductase, malondialdehyde (MDA), thiobarbituric acid (TBA), and 4-vinylpyridine (4-VP) were supplied by Sigma-Aldrich Chemical (St. Louis, Missouri, USA). Ammonium molybdate and hydrogen peroxide (H2O2) were purchased from Ajax Finechem (Melbourne, Australia). Trichloroacetic acid (TCA) was a product of Loba Chemie (Mumbai, India). All other laboratory chemicals were of the highest purity from commercial suppliers.
Hydrogen peroxide h2o2
Manufactured by Thermo Fisher Scientific
Sourced in United States, Australia, Germany, United Kingdom, Belgium, India
Hydrogen peroxide (H2O2) is a chemical compound that is a colorless liquid with a slightly pungent odor. It is a strong oxidizing agent and is commonly used as a bleaching agent, disinfectant, and in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Xanthine oxidase, by Merck Group (4 mentions)
4 vinylpyridine 4 vp, by Merck Group (3 mentions)
Formic acid hcooh, by Thermo Fisher Scientific (2 mentions)
Ethyl acetate ch3cooc2h5, by RCI Labscan (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (2 mentions)
Nitroblue tetrazolium chloride, by Merck Group (2 mentions)
Catalase, by Merck Group (2 mentions)
Streptozotocin (stz), by Merck Group (2 mentions)
Mitomycin c, by Thermo Fisher Scientific (2 mentions)
Tunicamycin, by Merck Group (2 mentions)
Ifn γ, by Thermo Fisher Scientific (2 mentions)
Celltiter glo luminescence cell viability assay, by Promega (2 mentions)
Murine recombinant il 1β, by BioLegend (2 mentions)
Genistein, by Merck Group (2 mentions)
Quercetin dihydrate, by Merck Group (2 mentions)
Isoflavone kaempferol, by Merck Group (2 mentions)
Reduced glutathione, by Merck Group (2 mentions)
Oxidized glutathione, by Merck Group (2 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
56 protocols using hydrogen peroxide h2o2
1
Evaluation of Plumbagin's Antioxidant Potential
2
Valorization of Used Palm Oil
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Used palm oil for preparing recycled palm oil (RPO) was purchased from a Nonthaburi local market, Nonthaburi province (acid value of 1.41 mg KOH/g and iodine value of 40.1 mg I2/g). The used palm oil was first filtered before modification. Hydrogen peroxide (H2O2, 35%) and sodium hydrogen carbonate (NaHCO3, >90%) were purchased from Ajax Finechem (Sydney, Australia). Formic acid (HCOOH, 98%) was purchased from Fisher Chemical (Shanghai, China). Ethyl acetate (CH3COOC2H5, >99%) was purchased from RCI Lab-Scan Limited (Bangkok, Thailand). Polymeric diphenylmethane diisocyanate (P-MDI, 31.5% NCO content, functionality = 2.7) was purchased from BASF (Ludwigshafen, Germany). T-12 (dibutyltin dilaurate, 95%) was purchased from Fluka Chemie AG CH-9471 Company (Darmstadt, Germany). Dabco 33LV (33% triethylene diamine in propylene glycol) was purchased from Evonilk Goldschmidt GmbH (Essen, Germany). Silicone surfactant (TEGOSTAB® B8110) was obtained from Gold-Schmidt (Berlin, Germany). Dried water hyacinth was purchased from Suphanburi province, Thailand. Commercial polyurethane foam, Polyurethane foam/glass fiber, and polyester foam were purchased from a convenience store, Nonthaburi province, Thailand.
3
Biodegradable Foam Composite Preparation
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Non-glutinous rice flour (Erawan brand) was purchased from Do Heng Rice Vermicelli Factory Co., Ltd. (Nakhon Prathom, Thailand). Baking powder double-acting formula, containing 30% of sodium bicarbonate (NaHCO3), 24% of disodium pyrophosphate (Na2H2P2O7) and 16% of monocalcium phosphate (Ca(H2PO4)2), was purchased from Best Food brand (Chachoengsao, Thailand). Polyvinyl alcohol (PVOH), a moisture resistance, with a molecular weight of 72,000 g/mol was purchased from Krungthepchemi Co., Ltd. (Bangkok, Thailand). Used palm oil for preparing recycled palm oil (RPO) was purchased from a Nonthaburi local market, Nonthaburi province (acid value of 1.41 mgKOH/g and iodine value of 40.1 mg I2/g). The used palm oil was first filtered before modification. Hydrogen peroxide (H2O2, 35%) was purchased from Ajax Finechem (Sydney, Australia). Formic acid (HCOOH, 98%) was purchased from Fisher Chemical (Shanghai, China). Ethyl acetate (CH3COOC2H5, >99%) was purchased from RCI Lab-Scan Limited (Bangkok, Thailand). Distilled water, purchased from Yod Nam Distilled Water (Nakhon Prathom, Thailand), was used to prepare gelatinized starch before foam preparation. All ingredients were dried before use.
4
Oxidative Stress Biomarker Analysis
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Trichloroacetic acid (TCA), thiobarbituric acid (TBA), phosphotungstic acid, tetraethoxypropane, nicotinamide adenine dinucleotide (NADH) and phenazine methosulfate (PMS), superoxide dismutase (SOD) standards, ethylenediaminetetraacetic acid (EDTA), nitro blue tetrazolium (NBT), phosphate-buffered saline (PBS), and 1% Triton X-100 were purchased from Sigma–Aldrich (St. Louis, MO, USA). Hydrochloric acid (HCl), acetic acid, sulfuric acid (H2SO4), magnesium chloride (MgC12), sodium hydroxide (NaOH), n-butanol, and hydrogen peroxide (H2O2) were purchased from Fisher Scientific (Chicago, IL, USA). The iron standard solution for AAS was purchased from Fluka (Buchs, Switzerland).
5
Measuring Oxidative Stress in Cell Lines
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Cells of each genotype were plated at 1.5x104 per well of a 96 well plate overnight. The next day, cells were incubated with 1.4 mM of hydrogen peroxide (H2O2) (Fisher Scientific, Fair Lawn, NJ) for 24 h at 33°C. The percentage of live cells was determined using the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit (Promega, Madison, WI). These experiments were repeated with two different isolation of LSEC with similar results.
6
Lindane Degradation via Oxidation
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Lindane (C6H6Cl6, 97%), sodium persulfate and titanium(iv ) isopropoxide (TTIP, 97%) were purchased from Sigma-Aldrich. Hydrogen peroxide (H2O2, 50% v/v) was obtained from Fisher Scientific. Commercial grade plastic bottles (100 mL) were used for the collection of water samples. All the chemicals were used as received. Milli-Q water (resistivity = 18.2 MΩ cm) was used for preparation of standard solutions of lindane while obtaining the calibration curve.
7
Pharmacological Modulation of Cellular Pathways
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FAC (Fisher Scientific) was dissolved in tissue culture-grade PBS and utilized at a final concentration of 100 or 250 μM. We have used FAC with treatment times of between 1, 6, 18, 24 and 48 h in the various Figures depending on the functional outcome assessed. U0126 was dissolved in DMSO (Cell Signaling Technology) and used at a final dose of 10 μM. Ru360 (Fisher Scientific) was dissolved in de-oxygenated tissue-culture grade water and utilized at a final concentration of 10 μM. OMA (sodium salt) (Cayman Chemicals) was dissolved in PBS and used at a final concentration of 5 mM. Hydrogen peroxide (H2O2; Fisher Scientific) was used at a final concentration of 100 μM. HTF (R&D Systems) was dissolved in PBS and utilized at concentrations from 0.0001 μg/ml to 100 mg/ml.
8
Catalase and Oxidase Activity Assay
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Catalase enzyme activity was confirmed with 3% v/v hydrogen peroxide (H2O2) (Fisher Chemical) (Taylor and Achanzar, 1972 (link)), and oxidase reagent (1% w/v tetramethyl-p-phenylenediamine dihydrochloride) (Acros Organics) was used for the oxidase test (Kovacs, 1956 (link)). Both catalase and oxidase test were done according to established protocols.
9
Antioxidant Peptide Evaluation Protocol
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Reduced L-glutathione (GSH), fatty acid free bovine serum albumin (BSA), potassium phosphate, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), diethylenetriaminepentaacetic acid (DETAPAC), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl-sulfoxide (DMSO), sodium azide (NaN3), glutathione reductase (GR), nicotinamide adenine dinucleotide phosphate (NADPH), cumene hydroperoxide, catalase, nitroblue tetrazolium chloride (NBT), xanthine oxidase, 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), 5-sulfosalicylic acid dehydrate, and 96-well and 60-mm tissue culture plates were purchased from Sigma Aldrich (Oakville, ON, Canada). Hydrogen peroxide (H2O2) and fluorescein were from Fisher Scientific Co., (Nepean, ON, Canada). Bathocuproine disulfonic acid (BCS) and xanthine were purchased from MP Biomedical (Solon, OH, USA) while 2,2′-azobis(2-methylpropionamidine) dihydrochloride (AAPH) was from Wako Chemicals USA Inc., (Richmond, VA, USA). Peptides: FNDRLRQGQLL (P1), GLVYIL (P2), GQTV (P3), GQTVFNDRLRQGQLL (P4), YHNAP (P5), YHNAPGLVYIL (P6), and DVNNNANQLEPR (P7) were synthesized by GenScript (Piscataway, NJ, USA) at a purity of more than 95%.
10
Cytotoxicity Screening of Compounds
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Cells were seeded in 96-well white plate (50,000 cells / well) for overnight culture with or without thapsigargin, tunicamycin, streptozotocin (Sigma-Aldrich), mitomycin C (Fisher Scientific), murine recombinant IL-1β (BioLegend) and IFN-γ (Peprotech), or hydrogen peroxide (H2O2, Fisher Scientific) at the indicated concentrations. Cell viability was assessed after 24 h using the CellTiter-Glo luminescence Cell Viability Assay (Promega).
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