Tissues were fixed in 10% buffered formalin and embedded in paraffin. Paraffin sections of tumor tissues were prepared, dewaxed by dimethyl-benzene, and hydrated with different concentrations of ethanol (100%, 95%, 85%, 70%, and 50%). The following steps were performed: blocking of endogenous peroxidase activity in 3% H2O2 solution, unmasking of the antigenic epitope with citrate buffer, incubation with blocking buffer for blocking, and incubation with primary antibody (VEGF (Bioss, BS1665R), CD34 (Bioss, BS5085R), TGF-β1(Bioss, BS0086R), IL-10 (Bioss, BS6761R), and EGFR (Bioss, BS1007R)) and secondary antibody (horseradish peroxidase-labeled goat rabbit IgG (H + L) (Beyotime, A0208)). Lastly, the DAB (Beyotime) substrate solution was applied to reveal the color of antibody staining. Images were acquired by an Motic BA400 microscope (Motic, China). The expression of cytokines was analyzed by Image-Pro Plus 6.0 (Media Cybernetics, USA).
Bs1007r
Manufactured by Bioss Antibodies
Sourced in United States
The BS1007R is a laboratory equipment product. It is a piece of equipment used for scientific research and analysis. The core function of the BS1007R is to perform a specific task or procedure in a laboratory setting. No further details about its intended use or capabilities are provided.
Automatically generated - may contain errors
Lab products found in correlation
Ba400 microscope, by Motic (2 mentions)
Bs1665r, by Bioss Antibodies (2 mentions)
Bs5085r, by Bioss Antibodies (2 mentions)
Horseradish peroxidase labeled goat rabbit igg h l, by Beyotime (2 mentions)
Bs 0086r, by Bioss Antibodies (1 mentions)
Bs 6761r, by Bioss Antibodies (1 mentions)
Image pro plus 6, by Media Cybernetics (1 mentions)
Ab3516, by Abcam (1 mentions)
3 protocols using bs1007r
1
Immunohistochemical Analysis of Tumor Markers
2
Cardiac Protein Expression Analysis
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The homogenization of the samples, protein measurements, electrophoresis, primary and secondary antibody incubation, and quantification were performed following our previously established protocols (Gergs et al. 2009 (Gergs et al. , 2019a,b;,b; Boknik et al. 2018) (link). First antibodies were rabbit polyclonal anti-calsequestrin (CSQ) antibody, #ab3516, Abcam, Cambridge, UK (diluted 1:20000), rabbit polyclonal anti-DRD1, #bs-1007R, Bioss, Woburn, MA, USA (dilution 1:500), and rabbit polyclonal anti-phospho-troponin I (P-TnI) antibody, #4004, Cell Signaling Technology Europe, Leiden, The Netherlands (diluted 1:5000).
As controls in some Western blotting experiments, cardiac homogenates from D 1 receptor knock-out mice were used. Cardiac samples of D 1 knock-out mice were kindly provided by Jean-Antoine Girault, Insern Research Director; Institute du Fer à Moulin, Paris, France.
As controls in some Western blotting experiments, cardiac homogenates from D 1 receptor knock-out mice were used. Cardiac samples of D 1 knock-out mice were kindly provided by Jean-Antoine Girault, Insern Research Director; Institute du Fer à Moulin, Paris, France.
3
Immunohistochemical Analysis of Tumor Biomarkers
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Tissues were fixed in 10% buffered formalin and embedded in paraffin. Paraffin sections of tumor tissues were prepared, dewaxed by dimethyl-benzene, and hydrated with different concentrations of ethanol (100%, 95%, 85%, 70%, and 50%). Subsequently, the following steps were performed: blocking of endogenous peroxidase activity in 3% H2O2 solution, unmasking of the antigenic epitope with citrate buffer, addition of blocking buffer for blocking, and addition of primary antibody VEGF (Bioss, BS1665R, 1 : 1000), CD34 (Bioss, BS5085R, 1 : 1000), and EGF (Bioss, BS1007R, 1 : 1000) and a secondary antibody (horseradish peroxidase-labeled goat rabbit IgG [H + L]; Beyotime, A0208). Finally, DAB substrate solution was applied to reveal the staining. Images were acquired using a Motic BA400 microscope (Motic, China).
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