Ni2 nitrilotriacetic acid sepharose superflow

Mouse hippocampal HT-22 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum and antibiotics (100 μg/ml streptomycin, 100 μg /ml penicillin) at 37°C in a humidity chamber with 5% CO₂ and 95% air.
Ni²⁺-nitrilotriacetic acid Sepharose Superflow was purchased from Qiagen (Valencia, CA, USA). PD-10 columns were purchased from Amersham (Braunschweig, Germany). The indicated primary and β-actin antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA) and Santa Cruz Biotechnology (Santa Cruz, CA, USA). An enzyme-linked immunosorbent assay (ELISA) kit for hexa histidine was obtained from Cloud-Clone Corp. (Houstern, TX, USA). Unless otherwise stated, all other agents were of the highest grade available.

Ni²⁺-nitrilotriacetic acid Sepharose Superflow was purchased from Qiagen (Valencia, CA, USA). PD-10 columns were purchased from Amersham (Brauncschweig, Germany). Fetal bovine serum (FBS) and antibiotics (streptomycin and penicillin) were obtained from Gibco BRL (Grand Island, NY, USA). Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Lonza/BioWhittaker (Walkersville, MD, USA). 2′,7′-Dichlorofluorescein diacetate (DCF-DA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Histidine antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The indicated antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). Unless otherwise stated, all other agents were of the highest grade available.
Mouse hippocampal HT-22 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% Fetal bovine serum (FBS) and antibiotics (100 μg/mL streptomycin, 100 μg /mL penicillin) at 37°C in a humidity chamber with 5% CO₂ and 95% air.