The largest database of trusted experimental protocols

4 protocols using foetal calf serum

1

Capripox Virus Isolation in Vero Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture was performed using Vero cells as recommended by the OIE protocol [18 ]. Briefly, 102 positive capripox virus PCR samples were inoculated to a 25 cm3 cell culture flask (Corning, Mediatech Inc., Virginia, USA) of 80% confluent monolayer for penetration at 37 °C for 2 h, then washed thrice where maintenance medium was added with 2% foetal calf serum (Corning, Corning, Mediatech Inc., Virginia, USA) and 1% antibiotics (Gibco, USA). The cultures were incubated at 37 °C with 5%CO2 with frequent changing of medium every 2 days. Cytopathic effect (CPE) was recorded daily under an inverted microscopic (Zeiss Axiovert 4 °C, Germany) for 14 days. Negative culture was considered when there was absence of CPE following two or more blind passages.
+ Open protocol
+ Expand
2

Quantifying Endocannabinoid Signaling Molecules

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dulbecco’s modified Eagle’s medium, foetal calf serum and penicillin/streptomycin were from Corning (Corning, NY, USA). Bovine serum albumin (BSA) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Sphingosine-1-phosphate (S1P, cod.62570) and methanandamide (N-(2-hydroxy-1R-methylethyl)-5Z,8Z,11Z,14Z-eicosatetraenamide, cod.157182-49-5) were from Cayman Chemicals (Ann Arbor, MI, USA); N-arachidonoyl-ethanolamine (anandamide (AEA), cod. A0580) and 2-arachidonoil-glycerol (2-AG, cod. A8973) were from Sigma-Aldrich (St. Louis, MO, USA). AEA-d8 and 2-AG-d8 were purchased from Cayman Chemicals (Ann Arbor, MI, USA) and were used as internal standards. The selective antagonists of CB1 SR141716A (SR1, cod. SML0800), of CB2 SR144528 (SR2, cod. SML1899) and of GPR55 ML193 trifluoroacetate (ML193, cod. SML1340) were from Sigma-Aldrich (St. Louis, MO, USA). The TRPV1 selective antagonist 5′-iodoresiniferatoxin (I-RTX, cod. 1362) was from TOCRIS (Bristol, UK). For molecular biology studies RevertAid H Minus First Strand cDNA Synthesis Kit, from Thermo Scientific (Waltham, MA, USA), and SensiFASTTM SYBR Lo-ROX kit, from Bioline (London, UK) were used.
+ Open protocol
+ Expand
3

AsiDNA Treatment Effects on HCT116-DNhTERT

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCT116-DNhTERT were grown in McCoy’s 5A (modified) medium with GlutaMAX (Gibco, Dublin, Ireland), supplemented with 10% Foetal Calf Serum (Corning, Tewksbury, MA, USA) and 1% penicillin/streptomycin (Gibco). Cells were cultured in 10 cm Ø plates and passaged ≈ every 3–4 days during the entire course of the experiment. Population Doublings (PDs) were calculated at every passage using the following equation: [log (total number of cells counted at day of passage)—log (number of cells initially seeded at previous passage)]/log2. Cumulative Population Doubling is the total number of PDs at a given day point. At each reseeding the remaining cells were pelleted and snap-frozen for further analysis. Treated cells were grown in presence of 10 μg/ml of AsiDNA and the treatment was renewed at every passage (every 3–4 days).
+ Open protocol
+ Expand
4

Murine Macrophage Polarization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow mononuclear (BMMNC) cells were collected by flushing the femurs and tibias from mice with PBS (J.T Baker, Phillipsburg, NJ, USA) in pH 7.4, containing 0.5% bovine serum albumin (BSA) (Sigma). The mononuclear cells were obtained after Ficoll‐paque™ PLUS (GE Healthcare, Uppsala, Sweden) density gradient centrifugation. Briefly, M1 was induced by incubating isolated mononuclear cells with M‐CSF (50 ng/mL; PEPROTECH, Rocky Hill, NJ, USA) for 7 days in RPMI 1640 (Corning, Manassas, VA, USA) supplemented with 10% foetal calf serum (PAA, Pasching, Austria), followed by LPS (1 μg/mL) treatment for 2 hours. M2 was induced by incubating mononuclear cells with 50 ng/mL M‐CSF for 7 days, followed by polarization with 10 ng/mL IL‐4 (CELL Guidance System, St. Louis, MO, USA) or 50 μmol/L baicalin (Tokyo Chemical Industry) for 24 hours. The results from BMMNC are only presented in Data S2 and S3.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!