Wright giemsa staining solution

Manufactured by Merck Group

Sourced in United States

The Wright-Giemsa staining solution is a laboratory reagent used for the differential staining of blood cells and other cellular components. It is a combination of the Wright stain and the Giemsa stain, which are commonly used in hematology and cytology. The solution provides a standardized method for staining and visualizing cellular structures, enabling the identification and analysis of various cell types.

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Giemsa (790 citations) Giemsa solution (315 citations) Wright-Giemsa (43 citations) Giemsa staining solution (36 citations) Giemsa staining (32 citations) Wright-Giemsa solution (16 citations) Wright-Giemsa staining (8 citations) Wright staining (4 citations) Wright solution (3 citations)

8 protocols using wright giemsa staining solution

Cell Preparation and Wright-Giemsa Staining

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Approximately 10,000 of cytospin prepared cells were air dried, fixed in 100% methanol for 1 min, stained in 100% in Wright-Giemsa staining solution (Sigma-Aldrich St. Louis, Missouri, USA) for 90 s, washed two times in deionized water, and air dried.

Brzezinka K., Nevedomskaya E., Lesche R., Steckel M., Eheim A.L., Haegebarth A, & Stresemann C. (2019). Functional diversity of inhibitors tackling the differentiation blockage of MLL-rearranged leukemia. Journal of Hematology & Oncology, 12, 66.

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EML Cell Differentiation and Wright-Giemsa Staining

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Blood progenitor EML cells (ATCC CRL-11691) were cultured and maintained as described previously [26 (link)]. Multipotent EML cell population was stimulated with either EPO (to differentiate into erythroid cells), GM-CSF/IL-3 and ATRA (to obtain myeloid cells), or a mixture of all cytokines for the “combined” treatment as previously reported [4 (link),26 (link)]. Wright-Giemsa staining was performed with some modification following a reported protocol [27 (link)]. In brief, 60,000 cells in 250 μl of PBS + 1% FBS buffer were cytospun at 350 rpm for 5 min per slide and allowed to air dry for 10 min. Slides were subjected to five 1-second dips in methanol, followed by Wright-Giemsa staining solution (0.4% [w/v], Sigma). After a final rinse with water, slides were allowed to air dry for 30 min. Colored phase contrast images were obtained using a Zeiss Axiovert 200M microscope.

Mojtahedi M., Skupin A., Zhou J., Castaño I.G., Leong-Quong R.Y., Chang H., Trachana K., Giuliani A, & Huang S. (2016). Cell Fate Decision as High-Dimensional Critical State Transition. PLoS Biology, 14(12), e2000640.

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Cell Morphology Analysis of K562 Cells

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Proliferating or differentiating K562 cells were attached to slides using TXT3 cytospin (Nasco Korea) at 900 × g for 5 min and stained with Wright-Giemsa staining solution (Sigma, 45253 and 32884). Cell morphology, including cell size and polynucleation, was analyzed by microscopy and ImageJ software. At least 290 cells in randomly separated fields were counted and expressed as relative cell size or percentage of the total cell number in these fields.

Jo J.H., Park J.U., Kim Y.M., Ok S.M., Kim D.K., Jung D.H., Kim H.J., Seong H.A., Cho H.J., Nah J., Kim S., Fu H., Redon C.E., Aladjem M.I, & Jang S.M. (2023). RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter. Cell Communication and Signaling : CCS, 21, 219.

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Antimalarial Drug Screening Protocol

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Dimethyl sulfoxide (DMSO)(density 1.10 g/mL) was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), whereas 1,2-dimethyl-3-hydroxypyridin-4-one, DFP or GPO-L-One^® (MW = 139), was kindly donated by the Institute of Research and Development, Government Pharmaceutical Organization, Bangkok, Thailand. Additionally, 1-octanol (MW = 130.23, CAS-No 111–87–5), Wright–Giemsa staining solution, lysis buffer, fetal bovine serum (FBS) and pyrimethamine (PYR, MW = 248.7) were purchased from Sigma-Aldrich Chemicals Company (St. Louis, MO, USA). Calcein acetoxymethyl (CA-AM, 1 mg/mL in DMSO, Catalogue Number C3099), 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT, Catalogue Number M6494), SYBR Green I nucleic acid gel-stain (10,000× concentrate in DMSO, Catalogue Number S7563) and SYTO^®61 red-fluorescent nucleic acid dye (Catalogue Number S11343) were purchased from Invitrogen, Molecular Probes (Thermo Fisher Scientific, Waltham, MA, USA). RPMI-1640 (Gibco^®Invitrogen) incomplete medium and phosphate-buffered saline (PBS) were purchased from Life Technologies Corporation (Carlsbad, CA, USA.)

Maneekesorn S., Chuljerm H., Koonyosying P., Uthaipibull C., Ma Y, & Srichairatanakool S. (2021). Identifying a Deferiprone–Resveratrol Hybrid as an Effective Lipophilic Anti-Plasmodial Agent. Molecules, 26(13), 4074.

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Isolation and Characterization of Erythroid Progenitor Cells

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The differentiated cells from a coculture system were harvested by manual picking and treating with 2 mg/ml Collagenase IV incubation for 30 minutes, and then with 0.25% Trypsin‐EDTA (Life Technologies) for 20 minutes. The single cells were counted and seeded at 1–2 × 10⁵ cell/ml into Methocult H4434 medium (StemCell Technologies) specified for CFC assay according to the manufacturer's protocol. The cells were maintained at 37°C, 5% CO₂ and the pattern of colony forming units (CFUs) was examined at day 12–13. At day 16, burst‐forming unit erythroid colonies were identified and picked for CD235a (Glycophorin A [GPA]) positive cell selection by the MACS (Miltenyi Biotech, San Diego, CA, http://www.miltenyibiotec.com) according to the manufacturer protocols. The purified CD235a positive cells were used for determining hemoglobin chain expression pattern. The cell morphology was evaluated by being spun onto a glass slide using cytocentrifugation (Shandon Cytospin 4; Thermo Scientific, Waltham, MA, http://www.thermoscientific.com) at 1,000 rpm for 5 minutes and stained with Wright‐Giemsa staining solution (Sigma‐Aldrich).

Phanthong P., Borwornpinyo S., Kitiyanant N., Jearawiriyapaisarn N., Nuntakarn L., Saetan J., Nualkaew T., Sa‐ngiamsuntorn K., Anurathapan U., Dinnyes A., Kitiyanant Y, & Hongeng S. (2017). Enhancement of β‐Globin Gene Expression in Thalassemic IVS2‐654 Induced Pluripotent Stem Cell‐Derived Erythroid Cells by Modified U7 snRNA. Stem Cells Translational Medicine, 6(4), 1059-1069.

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Proliferating or Differentiating K562 Cell Morphology

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Proliferating or differentiating K562 cells were attached to slides using TXT3 cytospin (Nasco Korea) at 900×g for 5 min and stained with Wright-Giemsa staining solution (Sigma, 45253 and 32884). Cell morphology, including cell size and polynucleation, was analyzed by microscopy and ImageJ software. At least 290 cells in randomly separated fields were counted and expressed as relative cell size or percentage of the total cell number in these fields.

Jo J.H., Ok S.M., Kim D.K., Kim Y.M., Park J.U., Jung D.H., Kim H.J., Seong H.A., Cho H.J., Nah J., Kim S., Fu H., Redon C.E., Aladjem M.I, & Jang S.M. (2023). RepID represses megakaryocytic differentiation by recruiting CRL4A-JARID1A at DAB2 promoter. Research Square.

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In vitro Hepatoprotective Assays

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Accordingly, 1-butanol, butylated hydroxytoluene (BHT), dimethyl sulfoxide (DMSO), heme iron reagent, pyrimethamine (PYR), 1, 1, 3, 3-tetramethoxypropane (TMP), thiobarbituric acid (TBA), and Wright−Giemsa staining solution were purchased from Sigma-Aldrich Chemicals, Company (St. Louis, MO, USA). DFP (1, 2-dimethyl-3-hydroxypyridin-4-one) was provided by the Institute of Research and Development, Government Pharmaceutical Organization, Bangkok, Thailand. Enzyme assay kits for AST, ALT, and ALP, along with a colorimetric assay reagent for albumin, were purchased from BIOLABO (Les Hautes Rives, Maizy, France).

Chuljerm H., Maneekesorn S., Punsawad C., Somsak V., Ma Y., Ruangsuriya J., Srichairatanakool S, & Koonyosying P. (2022). Deferiprone−Resveratrol Hybrid, an Iron-Chelating Compound, Acts as an Antimalarial and Hepatoprotective Agent in Plasmodium berghei-Infected Mice. Bioinorganic Chemistry and Applications, 2022, 3869337.

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Ortho-topolin riboside modulates STAT3 signaling

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Ortho-topolin riboside (oTR, purity >99%) was obtained from OlChemim GmbH (Czech Republic). RPMI 1640 and fetal bovine serum (FBS) were obtained from GIBCO (USA). Anti-STAT3, anti-phospho-STAT3^Y705, anti-JAK2, anti-phospho-JAK2^Y1007/1008, anti-phospho-SHP-1^Tyr564, anti-phospho-SHP-2^Tyr542, anti-SHP-1, anti-SHP-2, and β-actin were obtained from Cell Signaling (USA). Wright-Giemsa staining solution was obtained from Sigma-Aldrich Corporation (USA). Penicillin-streptomycin, Cell Counting Kit-8 (CCK-8), and phosphatase inhibitor complex were obtained from the Beyotime Institute of Biotechnology (Beijing, China). Anti-human CD11b-PE was obtained from eBioscience (USA).

Wang L., Cheng J., Lin F., Liu S., Pan H., Li M., Li S., Li N, & Li W. (2019). Ortho-Topolin Riboside Induced Differentiation through Inhibition of STAT3 Signaling in Acute Myeloid Leukemia HL-60 Cells. Turkish Journal of Hematology, 36(3), 162-168.

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