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2 protocols using sodium acetate 3 hydrate

1

Antioxidant Compounds Evaluation Protocol

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All chemicals and reagents were of analytical grade. Ethanol, methanol, gallic acid, rutin, trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), Folin-Ciocalteu reagent, ABTS (2,2′-azino-di(3-ethylbenzothiazoline-6-suslfonic acid), TPTZ (2,4,6-tri(2-pyridyl)-S-triazine), DPPH (2,2-diphenyl-1-picrylhydrazyl), sodium carbonate, sodium acetate 3-hydrate, potassium persulfate, acetic acid, hydrochloric acid, iron(III) chloride hexahydrate, sodium hydroxide and Dulbecco’s Modified Eagle Medium (DMEM), foetal bovine serum (FBS), penicillin-streptomycin solution, resazurin sodium salt, and dimethyl sulfoxide (DMSO, ≥99.9%) were obtained from Sigma-Aldrich (Barcelona, Spain).
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2

Spectrophotometric Analysis of Metal Ions

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The reagents used in the studies were pure sodium silicate neutral solution and H2SO4, 95–97% expertQ, ISO, from Scharlau (Scharlab S.L., Barcelona, Spain); Ludox TM-50 colloidal silica 50 wt.% suspension in H2O, 1,5-diphenylcarbazide ACS, and chromium (III) chloride hexahydrate, minimum 99%, from Sigma-Aldrich (Sigma-Aldrich, Steinheim, Germany); And iron sulphate 7-hydrate PA-ACS, potassium dichromate PA-ACS-ISO, 1,10-phenantroline 1-hydrate PA-ACS, acetic acid glacial purissimum, sodium acetate 3-hydrate (RFE, USP, BP Ph. Eur.) PRS-CODEX, HCl 37% PA-ACS-ISO, NaOH 98% ACS-ISO, and hydroxylammonium chloride (ACS, ISO) from Panreac (Panreac Química S.A., Barcelona, Spain). All solutions were produced with deionized water.
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