The human prostate cancer cell line, PC-3, was purchased from the RIKEN Cell Bank (Osaka, Japan). Cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and Penicillin-Streptomycin Mixture (FUJIFILM Wako Pure Chemicals). All cells were cultured in a humidified atmosphere containing 5% CO2 at 37 °C. The PrimeSurface® system (Sumitomo Bakelite, Tokyo, Japan) was used for three-dimensional spheroid formation. Cell suspensions were seeded onto a PrimeSurface 96U plate at 104 cells/well, and then cultured for 7 days.
Primesurface system
Manufactured by Sumitomo Bakelite
Sourced in Japan
The PrimeSurface® system is a specialized lab equipment developed by Sumitomo Bakelite. Its core function is to provide a consistent and uniform surface treatment, enabling enhanced adhesion and surface properties for a variety of materials.
Automatically generated - may contain errors
Lab products found in correlation
Flat bottomed dishes and plates, by Corning (2 mentions)
Penicillin streptomycin mixture, by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Dmem medium, by Fujifilm (1 mentions)
Penicillin, by Fujifilm (1 mentions)
Streptomycin, by Fujifilm (1 mentions)
Sw1353, by RIKEN Cell Bank (1 mentions)
3 protocols using primesurface system
1
3D Spheroid Culture of PC-3 Prostate Cancer Cells
2
LNCaP Cell Culture in 2D and 3D
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The human PC LNCaP cell line was purchased from the RIKEN Cell Bank (Osaka, Japan). They were cultured in RPMI 1640 medium supplemented with 10 nM dihydrotestosterone, 10% fetal bovine serum, and a penicillin–streptomycin mixture. The cells were cultured at 37 °C in a humidified atmosphere containing 5% CO2. Flat-bottomed dishes and plates (Corning, Corning, NY, USA) were used for the 2D cell culture [21 (link)]. The PrimeSurface® system (Sumitomo Bakelite, Tokyo, Japan) was used for the 3D spheroid culture. LNCaP cell suspensions were seeded onto a PrimeSurface 96U plate at 105 cells/well, and then cultured for 7 days.
3
Culturing Human Osteosarcoma and Chondrosarcoma Cells
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The human OS cell line MG‐63 and CS cell line SW‐1353 were purchased from the RIKEN Cell Bank. The lines were cultured in D‐MEM medium (FUJIFILM Wako Pure Chemical) supplemented with 10% fetal bovine serum (Sigma‐Aldrich Japan) and penicillin (100 units/mL)/streptomycin (100 μg/mL) mixture (FUJIFILM Wako Pure Chemical). All cells were cultured in a humidified atmosphere containing 5% CO2 in air at 37°C. Flat‐bottomed dishes and plates (Corning) were used in the two‐dimensional (2D) cell culture. The PrimeSurface® system (Sumitomo Bakelite) was used for 3D spheroid formation. Cell suspensions of MG‐63 and SW‐1353 were seeded onto a PrimeSurface 96 U plate at 104 cells/well and then cultured for 3‐4 d.
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