The RNAi lines that targeted ci (2125R-1), hib (9924R-1), rpb3 (7885R-1) and rpb7 (v100309) used in genetic screen were obtained from the National Institute of Genetics Stock Center (NIG), Japan and the Vienna Drosophila RNAi Center (VDRC), Austria. eyflp, hsflp and FRT82 flies were obtained from the Bloomington Stock Center. Flies of MS1096, GMR-Gal4, Gal80, HA-Ci−3P, Fg/Myc-SPOP, Fg-SPOPΔ3box, Fg-SPOPΔMATH, Fg-SPOPΔBTB, Myc-mouse-Gli2 have been described.40 (link),54 (link)
hibΔ6 is a hib mutant allele, whose coding sequence is replaced by the white gene. The transgenic flies of Fg-Rpb3, Fg-Rpb7, HA-Grim, HA-Hid, HA-Rpr, Fg-SPOPY87N, Fg-SPOPF102C, Fg-SPOPS119N, Fg-SPOPF125L, Fg-SPOPK129E, Fg-SPOPW131G and Fg-SPOPF133V were generated by injection of corresponding constructs into Drosophila embryos according to the methods described previously.55 (link) The parental strain for all germline transformations is w1118. All stocks used in this study were maintained and raised under standard conditions.
hibΔ6 is a hib mutant allele, whose coding sequence is replaced by the white gene. The transgenic flies of Fg-Rpb3, Fg-Rpb7, HA-Grim, HA-Hid, HA-Rpr, Fg-SPOPY87N, Fg-SPOPF102C, Fg-SPOPS119N, Fg-SPOPF125L, Fg-SPOPK129E, Fg-SPOPW131G and Fg-SPOPF133V were generated by injection of corresponding constructs into Drosophila embryos according to the methods described previously.55 (link) The parental strain for all germline transformations is w1118. All stocks used in this study were maintained and raised under standard conditions.