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Spectrum spectrometer

Manufactured by PerkinElmer
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The Spectrum spectrometer is a laboratory instrument used for spectroscopic analysis. It is designed to measure and analyze the spectrum of light, enabling the identification and quantification of various chemical compounds and materials.

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Lab products found in correlation

Zetasizer nano series, by Malvern Panalytical (1 mentions) Jsm 7800f, by JEOL (1 mentions) Tga 5500, by TA Instruments (1 mentions)

6 protocols using spectrum spectrometer

1

Characterization of Synthesized Nanoparticles

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The size distribution of synthesized nanoparticles was measured by field emission scanning electron microscopy (TE-SCAN VEGA instrument). Nanoparticle powder XRD measurement was performed using INEL EQuinox 3000 instruments with 40 kV, 30 mA, and 1.541874 Å wavelength. Ultraviolet–visible (UV–vis) absorption spectra of GelMA and TPO nanoparticles were measured by Biowave II spectrometer instruments. Also, it was performed on 1 mg/ml of TPO nanoparticles aqueous solution, and the light path length was 1 cm. PerkinElmer Spectrum spectrometer recorded the Fourier transform infrared (FTIR) spectrum of synthesized GelMA and TPO nanoparticles frequencies ranging from 400 to 4000 cm−1. Hydrogen-Nuclear Magnetic Resonance (H-NMR) was performed using Bruker BioSpin GmbH instrument.
Ghazali H.S., Askari E., Seyfoori A, & Naghib S.M. (2023). A high-absorbance water-soluble photoinitiator nanoparticle for hydrogel 3D printing: synthesis, characterization and in vitro cytotoxicity study. Scientific Reports, 13, 8577.
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2

Structural Analysis of Lipase-Functionalized CNF

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Fourier-transform infrared spectroscopy (FTIR) spectra were measured using a Perkin-Elmer Spectrum spectrometer in the range 4000–600 cm−1, in the ATR mode with 10 times accumulation. The UV-CD spectra (190–260 nm) were recorded on a JASCO J-810 CD instrument (JASCO) with a bandwidth of 0.5 nm and a scan speed of 50 nm min−1. All CD measurements were performed at 25 °C. All the spectra were corrected by subtracting a blank spectrum (without lipase). Each scan was repeated three times, and the spectra were then averaged out. Spectra manager software was used to analyze the protein CD spectra for determining the secondary structure fractions. Transmission electron microscopy (TEM, Tecnai 20, USA) was used to observe the changes in the surface texture of CNF before and after functionalization. For this, CNF samples were dispersed in demineralized water and a drop was placed on a copper grid and observed after drying.
Soni S., Dwivedee B.P, & Chand Banerjee U. (2018). Facile fabrication of a recyclable nanobiocatalyst: immobilization of Burkholderia cepacia lipase on carbon nanofibers for the kinetic resolution of a racemic atenolol intermediate. RSC Advances, 8(49), 27763-27774.
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3

Nanoparticle Characterization by SEM, DLS, and FTIR

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A Malvern Zetasizer NanoSeries was used to measure the size distribution and ζ-potential of the samples. In addition, SEM (JEOL JSM-7800F, Pleasanton, CA, USA) was used to evaluate the morphology of the nanoparticles. Samples were diluted 1 to 10 before deposition on an aluminum film. FTIR spectra were collected on a Spectrum spectrometer (Perkin Elmer Inc., Waltham, MA, USA) equipped with a single attenuated total reflectance (ATR) diamond set to a range of 400 cm−1 to 4000 cm−1 [31 (link)].
Luque-Alcaraz A.G., Velazquez-Antillón M., Hernández-Téllez C.N., Graciano-Verdugo A.Z., García-Flores N., Iriqui-Razcón J.L., Silvas-García M.I., Zazueta-Raynaud A., Moreno-Vásquez M.J, & Hernández-Abril P.A. (2022). Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction. Materials, 15(14), 4838.
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4

FT-IR Spectroscopy of Liquid Samples

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FT‐IR spectra were recorded on a PerkinElmer Spectrum spectrometer. The liquid sample in CHCl3/MCH (5:95, v/v) was drop‐casted and solvent was allowed to evaporate on the surface naturally.
Ghule N.V., La D.D., Bhosale R.S., Al Kobaisi M., Raynor A.M., Bhosale S.V, & Bhosale S.V. (2016). Effect of Amide Hydrogen Bonding Interaction on Supramolecular Self‐Assembly of Naphthalene Diimide Amphiphiles with Aggregation Induced Emission. ChemistryOpen, 5(2), 157-163.
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5

Characterization of DHTA and DHIA Powders

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FTIR spectra on DHTA and DHIA powders were collected by using a PerkinElmer Spectrum spectrometer between 4000 and 750 cm−1 at 1 cm−1 resolution. 1H NMR analyses were performed with DHTA samples dissolved in a solution (20:1 by volume) of DMSO-d6 and 40 wt % sodium deuteroxide NaOD in D2O and filtered through a 0.44 μm Nylon filter. DHIA 1H NMR samples were dissolved by using DMSO-d6. Samples for 1H NMR were run on a Bruker AVANCE III Nanobay 400 MHz NMR spectrometer. The molecular weights of DHIA and DHTA were found using SEC. DHTA and DHIA were measured against polystyrene standards in NMP with 30 mM LiBr and H3PO4 as the mobile phase. Thermogravimetric analysis (TGA 5500, TA Instruments) of DHTA and DHIA was performed over a temperature range of 25–600 °C by using a 10 °C min−1 temperature ramp, with an isothermal hold at 120 °C for 30 min to remove water.
Fraser A.C., Chew N.G., Hegde M., Liu F., Liu C.W., Coronell O, & Dingemans T.J. (2022). Linear versus Nonlinear Aromatic Polyamides: The Role of Backbone Geometry in Thin Film Salt Exclusion Membranes. ACS applied materials & interfaces, 14(31), 36143-36156.
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6

Infrared Spectroscopic Characterization

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Samples were characterized by Infrared (IR) spectroscopy with a 1000 FT-IR Perkin-Elmer Spectrum spectrometer in the 400–4000 cm−1 frequency range.
Hachem K., Faugeron C., Kaid-Harche M, & Gloaguen V. (2016). Structural Investigation of Cell Wall Xylan Polysaccharides from the Leaves of Algerian Argania spinosa. Molecules, 21(11), 1587.
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