miR-510 inhibitor, miRNA inhibitor negative control (NC inhibitor), SRCIN1 siRNA, and NC siRNA were designed and purchased from Guangzhou RiboBio Co., Ltd. (Guangzhou, P.R. China). Cells were seeded into six-well plates at a density of 50% confluence. After incubation overnight, transient transfection was conducted using Lipofectamine 2000 transfection reagent (Invitrogen; Thermo Fisher Scientific, Inc.) following the manufacturer’s protocols. After incubating for 6–8 h, the medium was replaced by RPMI-1640 or DMEM containing 10% FBS.
Mir 510 inhibitor
Manufactured by RiboBio
Sourced in China
The MiR-510 inhibitor is a laboratory product designed to inhibit the function of the microRNA-510 (miR-510). It is a tool used in research studies to investigate the role and effects of miR-510 in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000 transfection, by Thermo Fisher Scientific (1 mentions)
Nc sirna, by RiboBio (1 mentions)
Ncm460, by Shanghai Cell Bank (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)
Mimic nc, by RiboBio (1 mentions)
2 protocols using mir 510 inhibitor
1
miRNA Inhibitor Transfection Protocol
2
miR-510 Regulation in Colon Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human colon cancer cells, LoVo, CaCo-2, SW480, and HCT-116, and normal colonic mucosa cell NCM460 were obtained from Shanghai Cell Bank and cultured in DMEM medium (Thermo Fisher Scientific) with 10% fetal bovine serum (FBS) at 37°C in a 5% CO2 humidified incubator.
miR-510 mimic, miR-510 inhibitor, and corresponding negative controls (mimic NC and inhibitor NC) were purchased from RiboBio (Guangzhou, China) for the overexpression or knockdown of miR-510. The sequences were: 5′-UACUCAGGAGAGUGGCAAUCAC-3′ for miR-510 mimic, 5′-GUGAUUGCCACUCUCCUGAGUA-3′ for the miR-510 inhibitor, 5′- GGACCAAATCTCGAGATTTGG-3′ for mimic NC, 5′-UUCUCCGAACGUGUCACGUU-3′ for inhibitor NC. Cell transfection was performed with the Lipofectamine 2000 Reagent (Invitrogen, USA) following the manufacturers’ protocol.
miR-510 mimic, miR-510 inhibitor, and corresponding negative controls (mimic NC and inhibitor NC) were purchased from RiboBio (Guangzhou, China) for the overexpression or knockdown of miR-510. The sequences were: 5′-UACUCAGGAGAGUGGCAAUCAC-3′ for miR-510 mimic, 5′-GUGAUUGCCACUCUCCUGAGUA-3′ for the miR-510 inhibitor, 5′- GGACCAAATCTCGAGATTTGG-3′ for mimic NC, 5′-UUCUCCGAACGUGUCACGUU-3′ for inhibitor NC. Cell transfection was performed with the Lipofectamine 2000 Reagent (Invitrogen, USA) following the manufacturers’ protocol.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!