Uas cdc42v12

Manufactured by Bloomington Drosophila Stock Center

UAS-Cdc42V12 is a genetic tool used in Drosophila research. It is a transgenic construct that expresses a constitutively active form of the Cdc42 protein under the control of the UAS promoter.

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2 protocols using uas cdc42v12

Drosophila Genetic Manipulation Protocol

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The following stocks were used: pnr-GAL4 (Bloomington:3039), aPKC^ts (Guilgur et al., 2012 (link)), cdc42³ (Fehon et al., 1997 (link)), tub-GAL80^ts (Bloomington:7108), UAS-Dia^CA (Bloomington:27616), UAS-Cdc42^V12 (Bloomington:6287), neu-GMA (Edwards et al., 1997; Kunda et al., 2012 ), neu-GAL4 (Bellaïche et al., 2001 (link)), Lifeact::GFP (Hatan et al., 2011 (link)), sqh^Ax3; Sqh::GFP (Monier et al., 2010 (link)), Cdc42^V5 (Fletcher et al., 2012 (link)), UAS-Tub::RFP (McGill et al., 2009 (link)), and UAS-Pbl::GFP (Somers and Saint, 2003 (link)). Vienna Drosophila Resource Center (VDRC) RNAi lines were used to silence the expression of the genes aPKC, arp3, diaphanous, pebble, and par-6, and a NIG-Fly library line was used for cdc42. For Flybase ID, see Supplemental Experimental Procedures.

Rosa A., Vlassaks E., Pichaud F, & Baum B. (2015). Ect2/Pbl Acts via Rho and Polarity Proteins to Direct the Assembly of an Isotropic Actomyosin Cortex upon Mitotic Entry. Developmental Cell, 32(5), 604-616.

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Drosophila Endocytic Trafficking Regulators

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All crosses and staging were performed at 25°C. Stocks are described in FlyBase (http://flybase.bio.indiana.edu). UAS-YFP-Rab11, UAS-YFP-Rab11^Q70L, UAS-YFP-Rab11^S25N, UAS-Rab11-RNAi, UAS-Cdc42^V12, UAS-YFP-Rab8^Q67L, UAS-YFP-Rab4, UAS-YFP-Rab14^Q97L, UAS-aPKC-RNAi, UAS-Rho1, Dia⁵ FRT40A, UAS-YFP-Rab5, GMR-gal4, Nuf^EY11342, Rho1^72F, Cdc42⁴ FRT19A, UAS-Sec8, Rab11^EP3017, UAS-Rok-CAT, UAS-LIMK-HA, UAS-MBS-RNAi, and Sqh^AX3; RLC-GFP were provided by the Bloomington Stock Center (Indiana University, Bloomington, IN); UAS-Rab5-RNAi was provided by the Vienna Drosophila RNAi Center (Vienna, Austria); UAS-Rok-RNAi and UAS-PKN-RNAi were provided by the National Institute of Genetics (Mishima, Japan); Shg^R69 FRT42D was provided by C. Micchelli (Washington University, St. Louis, MO); Par6^Δ226 FRT19A and UAS-aPKC^CAAX were provided by C. Doe (University of Oregon, Eugene, OR); Sec5^E10 FRT40A and UAS-Sec5 were provided by T. L. Schwarz (Harvard Medical School, Boston, MA); dRip11^d00190 was provided by the Exelixis Collection (Harvard Medical School); and UAS-Rho1 RNAi, UAS-Cdc42 RNAi, and UAS-Cdc42 were generated by our lab (Warner and Longmore, 2009a (link), b (link)).

Yashiro H., Loza A.J., Skeath J.B, & Longmore G.D. (2014). Rho1 regulates adherens junction remodeling by promoting recycling endosome formation through activation of myosin II. Molecular Biology of the Cell, 25(19), 2956-2969.

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