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Csii ef dhb venus

Manufactured by Addgene
Sourced in Spain

The CSII-EF-DHB-Venus is a lab equipment product. It is a plasmid that encodes the fluorescent protein Venus under the control of the EF1-alpha promoter and contains a Hygromycin resistance gene.

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2 protocols using csii ef dhb venus

1

Multimodal Cellular Imaging Toolkit

Check if the same lab product or an alternative is used in the 5 most similar protocols
pHIV-H2B-mRFP was purchased from Addgene (#18982) for stable H2B-RFP expression, FG12-GFP was kindly provided by the Soengas lab (CNIO, Spain) (Olmeda et al., 2017 (link)) for stable GFP expression, CSII-EF-DHB-Venus (sensor for Cdk2 activity) was kindly provided by the Spencer lab (University of Colorado, USA) for cell cycle analysis, and a pLifeact-mRuby lentiviral vector was kindly provided by the Moreau and Saltel laboratories (DR2 INSERM, France) as in (Juin et al., 2014 (link)) for imaging actin dynamics. CFP-YFP RhoA biosensor (Pertz et al., 2006 (link)), a CFP-YFP Cdc42 biosensor (Hanna et al., 2014 (link)), and a CFP-YFP Rac1 biosensor (Miskolci et al., 2016 (link)) were kindly provided by the Hodgson lab (AECOM, USA). Recombinant Human TGF-beta 2/TGFβ2 Protein was purchased from AbClonal (RP00452) and diluted according to manufacturer’s instructions. LY364947 (Selleck Chemical, S2805), an ATP-competitive inhibitor of TGFβR-I was diluted according to manufacturer’s instructions.
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2

Multimodal Cellular Imaging Toolkit

Check if the same lab product or an alternative is used in the 5 most similar protocols
pHIV-H2B-mRFP was purchased from Addgene (#18982) for stable H2B-RFP expression, FG12-GFP was kindly provided by the Soengas lab (CNIO, Spain) (Olmeda et al., 2017 (link)) for stable GFP expression, CSII-EF-DHB-Venus (sensor for Cdk2 activity) was kindly provided by the Spencer lab (University of Colorado, USA) for cell cycle analysis, and a pLifeact-mRuby lentiviral vector was kindly provided by the Moreau and Saltel laboratories (DR2 INSERM, France) as in (Juin et al., 2014 (link)) for imaging actin dynamics. CFP-YFP RhoA biosensor (Pertz et al., 2006 (link)), a CFP-YFP Cdc42 biosensor (Hanna et al., 2014 (link)), and a CFP-YFP Rac1 biosensor (Miskolci et al., 2016 (link)) were kindly provided by the Hodgson lab (AECOM, USA). Recombinant Human TGF-beta 2/TGFβ2 Protein was purchased from AbClonal (RP00452) and diluted according to manufacturer’s instructions. LY364947 (Selleck Chemical, S2805), an ATP-competitive inhibitor of TGFβR-I was diluted according to manufacturer’s instructions.
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