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Dp 72 microscope digital camera system

Manufactured by Olympus
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Sourced in Japan

The DP-72 is a microscope digital camera system from Olympus. It is designed to capture high-quality digital images from microscopes. The DP-72 features a high-resolution CMOS sensor and advanced image processing capabilities to produce detailed and accurate representations of microscopic samples.

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Lab products found in correlation

Cx41 microscope, by Olympus (7 mentions) Ab53931, by Abcam (2 mentions) M0897, by Agilent Technologies (2 mentions) Ab83404, by Abcam (2 mentions) Hpa028911, by Merck Group (2 mentions) Ish kit, by Thermo Fisher Scientific (2 mentions) Horseradish peroxidase conjugated secondary antibodies, by Nichirei Biosciences (1 mentions) 3 3 diaminobenzidine (dab), by Nichirei Biosciences (1 mentions)

8 protocols using dp 72 microscope digital camera system

1

Immunohistochemical Analysis of Cancer Tissue Samples

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Nasopharyngeal carcinoma, breast cancer and colon cancer biopsies were obtained from the Pathology Department of Xiangya Hospital. The nasopharyngeal tissue array was purchased from Pantomics (Richmond, CA, USA). IHC analysis of paraffin sections from nasopharyngeal, breast and colon tissues or xenograft samples was performed as previously described. The sections were incubated with antibodies as indicated. The images were surveyed and captured using a CX41 microscope (OLYMPUS, Tokyo, Japan) with a DP-72 Microscope Digital Camera System (OLYMPUS, Tokyo, Japan) and differentially quantified by two pathologists from Second Xiangya Hospital, Changsha, China.
Jia J., Shi Y., Chen L., Lai W., Yan B., Jiang Y., Xiao D., Xi S., Cao Y., Liu S., Cheng Y, & Tao Y. (2017). Decrease in Lymphoid Specific Helicase and 5-hydroxymethylcytosine Is Associated with Metastasis and Genome Instability. Theranostics, 7(16), 3920-3932.
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2

Immunohistochemical Analysis of Intestinal Organoids

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Paraffin-embedded intestinal organoids and mouse colon tissues were used. After deparaffinization and antigen retrieval, endogenous peroxidase activity was blocked by 3% hydrogen peroxide for 10 minutes. After blocking for 30 minutes, the sections were incubated overnight at 4°C with primary antibody, followed by incubation with horseradish peroxidase-conjugated secondary antibodies (Nichirei Bioscience, Tokyo, Japan) for 30 minutes. Probes were visualized using 3,3′-diaminobenzidine in the buffered substrate (Nichirei Bioscience). Images were acquired using the Olympus DP72 microscope digital camera system. The primary antibodies used are listed in Table 1.
Miyakawa Y., Otsuka M., Shibata C., Seimiya T., Yamamoto K., Ishibashi R., Kishikawa T., Tanaka E., Isagawa T., Takeda N., Kamio N., Imai K, & Fujishiro M. (2024). Gut Bacteria-derived Membrane Vesicles Induce Colonic Dysplasia by Inducing DNA Damage in Colon Epithelial Cells. Cellular and Molecular Gastroenterology and Hepatology, 17(5), 745-767.
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3

Immunohistochemistry of Lung Cancer

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Xiangya Hospital’s Department of Pathology confirmed and gave biopsies of lung cancer and related disorders. Previous literature describes the procedure for IHC examination of paraffin slices from lung cancer tissues. Two pathologists from Xiangya Hospital in Changsha, China, used a CX41 microscope (Olympus, Tokyo, Japan) with a DP-72 microscope digital camera system (Olympus, Tokyo, Japan) to record images of the paraffin sections, and differential quantification was conducted by two pathologists from Xiangya Hospital in Changsha, China.
Long Y., Guo J., Chen J., Sun J., Wang H., Peng X., Wang Z., Lai W., Liu N., Shu L., Chen L., Shi Y., Xiao D., Liu S, & Tao Y. (2023). GPR162 activates STING dependent DNA damage pathway as a novel tumor suppressor and radiation sensitizer. Signal Transduction and Targeted Therapy, 8, 48.
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4

Immunohistochemical Analysis of Lung Cancer

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The Department of Pathology at Xiangya Hospital verified and provided biopsies of lung cancer and associated diseases. The method used for IHC analysis of paraffin sections from lung cancer tissues can be found in previous literature. Images of the paraffin sections were captured with a CX41 microscope (Olympus, Tokyo, Japan) equipped with a DP-72 microscope digital camera system (Olympus, Tokyo, Japan), and differential quantification was performed by two pathologists from Xiangya Hospital, Changsha, China.
Ouyang L., Yan B., Liu Y., Mao C., Wang M., Liu N., Wang Z., Liu S., Shi Y., Chen L., Wang X., Cheng Y., Cao Y., Xiao D., Zhang L., Liu S, & Tao Y. (2020). The deubiquitylase UCHL3 maintains cancer stem-like properties by stabilizing the aryl hydrocarbon receptor. Signal Transduction and Targeted Therapy, 5, 78.
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5

Immunohistochemical Analysis of NPC Biomarkers

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NPC biopsies, validated by pathologist Dr. Desheng Xiao (Xiangya Hospital), were obtained from Pathology Department of Xiangya Hospital. The NPC tissue array was purchased from Pantomics (Richmond, CA, USA). Paraffin sections from NPC patient samples were firstly dewaxed and antigen retrieved in citrate buffer using a microwave for 15min. After cooling of the citrate buffer to room temperature, the sections were incubated with PBS (containing 5% bovine serum albumin (BSA), and 3% FBS) for 30 min, and subsequently incubated with HoxB3 (ab83404, Abcam), HoxB13 (ab53931, Abcam), HoxC8 (HPA028911, Sigma) or LMP1 (M0897, DAKO) primary antibody for 1 h. The slides were thoroughly washed three times with PBS (5%BSA, 3%FBS) solution for 10 min each and then incubated for 30 min with HRP- conjugated secondary antibody for 30 min at room temperature. The slides were thoroughly washed three times with PBS before using DAB. The images were surveyed and captured using a CX41 microscope (OLYMPUS, Tokyo, Japan) with the Microscope Digital Camera System DP-72 (OLYMPUS, Tokyo, Japan) and differentially quantified by two pathologists, Dr. Bo Li and Dr. Songqing Fan (The Second Xiangya Hospital, Changsha, China).
In situ hydridization (ISH) was performed using the EBERs HRP conjugated probe and DAB as substrate from ISH kit (Life technologies), according to the instructions of the manufacturers.
Jiang Y., Yan B., Lai W., Shi Y., Xiao D., Jia J., Liu S., Li H., Lu J., Li Z., Chen L., Chen X., Sun L., Muegge K., Cao Y, & Tao Y. (2015). Repression of Hox genes by LMP1 in nasopharyngeal carcinoma and modulation of glycolytic pathway genes by HoxC8. Oncogene, 34(50), 6079-6091.
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6

Immunohistochemical Analysis of NPC Biomarkers

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NPC biopsies, validated by pathologist Dr. Desheng Xiao (Xiangya Hospital), were obtained from Pathology Department of Xiangya Hospital. The NPC tissue array was purchased from Pantomics (Richmond, CA, USA). Paraffin sections from NPC patient samples were firstly dewaxed and antigen retrieved in citrate buffer using a microwave for 15min. After cooling of the citrate buffer to room temperature, the sections were incubated with PBS (containing 5% bovine serum albumin (BSA), and 3% FBS) for 30 min, and subsequently incubated with HoxB3 (ab83404, Abcam), HoxB13 (ab53931, Abcam), HoxC8 (HPA028911, Sigma) or LMP1 (M0897, DAKO) primary antibody for 1 h. The slides were thoroughly washed three times with PBS (5%BSA, 3%FBS) solution for 10 min each and then incubated for 30 min with HRP- conjugated secondary antibody for 30 min at room temperature. The slides were thoroughly washed three times with PBS before using DAB. The images were surveyed and captured using a CX41 microscope (OLYMPUS, Tokyo, Japan) with the Microscope Digital Camera System DP-72 (OLYMPUS, Tokyo, Japan) and differentially quantified by two pathologists, Dr. Bo Li and Dr. Songqing Fan (The Second Xiangya Hospital, Changsha, China).
In situ hydridization (ISH) was performed using the EBERs HRP conjugated probe and DAB as substrate from ISH kit (Life technologies), according to the instructions of the manufacturers.
Jiang Y., Yan B., Lai W., Shi Y., Xiao D., Jia J., Liu S., Li H., Lu J., Li Z., Chen L., Chen X., Sun L., Muegge K., Cao Y, & Tao Y. (2015). Repression of Hox genes by LMP1 in nasopharyngeal carcinoma and modulation of glycolytic pathway genes by HoxC8. Oncogene, 34(50), 6079-6091.
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7

Immunohistochemical Analysis of Skin Diseases

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Skin and related diseases biopsies, validated by pathologist Dr. Desheng Xiao (Xiangya Hospital), were obtained from Pathology Department of Xiangya Hospital. The skin tissue array was purchased from Pantomics (Richmond, CA, USA). IHC analysis of paraffin sections from skin tissues or xenograft samples was described previously [56 (link)]. The sections were incubated with antibodies as indicated. The images were surveyed and captured using a CX41 microscope (OLYMPUS, Tokyo, Japan) with the Microscope Digital Camera System DP-72 (OLYMPUS, Tokyo, Japan) and differentially quantified by two pathologists who were from the Second Xiangya Hospital, Changsha, China.
IKKα staining was considered positively by ascertaining cytoplasmic and nuclear expression. The determination result was obtained from semi-quantitative classification according to 10 more visual fields (×200). The slides were first scored as 0 (negative), 1 (buff), 2 (pale brown), and 3 (tan). Positive expression of IKKα were scored as 0 (negative), 1+ (< 10% of positively-staining tumor cells), 2+ (11-50% of positively-staining tumor cells), 3+ (50-75% of positively-staining tumor cells), and 4+ (> 75% of positively-staining tumor cells. Both the scores by multiply were regarded as the determination result.
Jia J., Shi Y., Yan B., Xiao D., Lai W., Pan Y., Jiang Y., Chen L., Mao C., Zhou J., Xi S., Cao Y., Liu S, & Tao Y. (2016). LGR5 expression is controled by IKKα in basal cell carcinoma through activating STAT3 signaling pathway. Oncotarget, 7(19), 27280-27294.
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8

Immunohistochemical Analysis of Glioma Biomarkers

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Gliomas and related diseases biopsies were validated and obtained from Department of Pathology in Xiangya Hospital. IHC analysis of paraffin sections from gliomas tissues was described previously 38 (link). The sections were incubated with antibodies as indicated. The images were surveyed and captured using a CX41 microscope (OLYMPUS, Tokyo, Japan) with the Microscope Digital Camera System DP-72 (OLYMPUS, Tokyo, Japan) and differentially quantified by two pathologists who were from the Xiangya Hospital, Changsha, China.
GSK3β and LRP6 staining were considered positively by ascertaining cytoplasmic staining, whereas LSH and E2F1 were considered positively by nuclear expression. The determination results were obtained from semi-quantitative classification according to 10 or more visual fields (×200). The slides were first scored as 0 (negative), 1 (buff), 2 (pale brown), and 3 (tan). Positive expression of LSH, E2F1, GSK3β or LRP6 was scored as 0 (negative), 1+ (<10% of positively-staining tumor cells), 2+ (11-50% of positively-staining tumor cells), 3+ (50-75% of positively-staining tumor cells), and 4+ (>75% of positively-staining tumor cells). Both the scores by multiply were regarded as the determination result.
Xiao D., Huang J., Pan Y., Li H., Fu C., Mao C., Cheng Y., Shi Y., Chen L., Jiang Y., Yang R., Liu Y., Zhou J., Cao Y., Liu S, & Tao Y. (2017). Chromatin Remodeling Factor LSH is Upregulated by the LRP6-GSK3β-E2F1 Axis Linking Reversely with Survival in Gliomas. Theranostics, 7(1), 132-143.
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