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β nicotinamide adenine dinucleotide reduced disodium salt hydrate

Manufactured by Merck Group
Sourced in United States

β-nicotinamide adenine dinucleotide reduced disodium salt hydrate is a coenzyme used in various biochemical reactions as an electron carrier. It is a derivative of nicotinamide adenine dinucleotide (NAD), a key cofactor in cellular metabolism.

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3 protocols using β nicotinamide adenine dinucleotide reduced disodium salt hydrate

1

Platelet Activation Assay Protocol

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UA and OA were purchased from Abcam (Cambridge, UK). Adenosine diphosphate (ADP) and collagen were from Chrono-log (Havertown, PA, USA). Thrombin, sodium pyruvate, β-nicotinamide adenine dinucleotide reduced disodium salt hydrate (NADH), menadione and dipyridamole were obtained from Sigma-Aldrich (St. Louis, MO, USA). Hematologic reagents for proThrombin time (PT) and activated thromboplastin time (aPTT) assays were purchased from Fisher Diagnostics (Middletown, VA, USA). Heparin was obtained from Choongwae Pharma (Seoul, Korea). All other chemicals used were of the highest purity available and were purchased from standard suppliers.
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2

Plasma Treatment of Aqueous Solutions

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β-Nicotinamide adenine dinucleotide reduced disodium salt hydrate (NADH), β-nicotinamide adenine dinucleotide sodium salt hydrate (NAD+), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), 1,4-hydroquinone, 1,4-benzoquinone, and L(+)-ascorbic acid sodium salt (vitamin C sodium salt) were purchased from Sigma-Aldrich (USA). H2O2, nitrate, and nitrite test strips were purchased from Bartovation LLC (New York, NY, USA). These strips were used for the detection of H2O2, NO2, and NO3 in water after the treatment with cold atmospheric pressure He-plasma jet. Time of the aqueous solution treatment was shown in Figure 3. Bottled ultra-high purity helium was purchased from Sexton Welding Supply (Huntsville, AL, USA).
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3

Metabolic Assays for Insulin Sensitivity

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Acetic acid, propionic acid and butyric acid were purchased from Sigma-Aldrich (Saint Louis, MO, USA) as well as bovine serum albumin fatty-acid free (BSA), human insulin solution, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), Triton X-100 (TX-100), β-nicotinamide adenine dinucleotide reduced disodium salt hydrate (NADH), sodium pyruvate, β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH), sulfosalicylic acid solution (SSA), EthylenediaminetetraAcetic acid tetrasodium salt dihydrate (EDTA), reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione reductase (GR), 2-vinylpyridine (2-VP), 5,5ʹ-Dithio-bis-(2-nitrobenzoic Acid) (DTNB), Potassium dihydrogen phosphate (KH2PO4), Dipotassium hydrogen phosphate (K2HPO4), human insulin solution, metformin, and menadione. Ethanol and the Pierce bicinchoninic acid (BCA) protein assay kit were purchased from ThermoFisher Scientific (Fremont, CA, USA). The Glucose Uptake-Glo assay kit was purchased from Promega (Leiden, The Netherlands).
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