Rabbit anti gapdh abs16

Unless otherwise noted, general reagents were obtained from Sigma (St. Louis, MO), cell culture reagents and electrophoresis/western blot supplies were from Invitrogen (Carlsbad, CA) and cell culture plasticware from Corning (Corning, NY). Monoclonal mouse anti-puromycin clone 12D10 [1 (link)] and rabbit anti-GAPDH (ABS16) were obtained from EMD-Millipore (Billerica, MA). Monoclonal mouse anti-cFLIP clone 7F10 was obtained from Enzo (Farmingdale, NY). Secondary antibodies, Goat anti-rabbit (800) and Goat anti-mouse (680) and blocking buffer were from LiCor (Lincoln, NE). Sources of other reagents: 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT; NSC 601519) from the NCI Drug Synthesis and Chemistry Branch; recombinant TRAIL ligand (168 amino acid TNF homologous extracellular domain—Peprotech). Caspase 8 assay kit (CaspaseGlo 8) was obtained from Promega (Madison, WI) and was used according the manufacturer’s directions.

2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT; NSC 601519) was obtained from the NCI Drug Synthesis and Chemistry Branch. Sources of other reagents included: recombinant TRAIL ligand (168 amino acid TNF homologous extracellular domain – Peprotech, Rocky Hill, NJ); Z-VAD-FMK (Promega, Madison, WI); cell culture media and additives (Cellgro, Hyclone, Sigma, or Invitrogen); BCA protein assay kits (Pierce/Thermo); puromycin (Invitrogen, Carlsbad, CA). Monoclonal mouse anti-puromycin clone 12D10 and rabbit anti-GAPDH (ABS16) were obtained from EMD-Millipore (Billerica, MA). Secondary antibodies: Goat anti-rabbit (800) and Goat anti-mouse (680) and blocking buffer were from LiCor (Lincoln, NE). Other reagents from Sigma (St. Louis, MO).