96 aqueous one solution proliferation assay system

Manufactured by Promega

The 96 AQueous One Solution Proliferation Assay System is a colorimetric method for quantifying cell proliferation and viability in a 96-well format. The assay utilizes a novel tetrazolium compound that is bioreduced by metabolically active cells, producing a colored formazan product that can be measured spectrophotometrically.

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Lab products found in correlation

Anti bcl xl, by Santa Cruz Biotechnology (3 mentions) Anti bcl 2, by Santa Cruz Biotechnology (3 mentions) Fitc annexin 5 apoptosis detection kit, by BD (3 mentions) Anti survivin, by Santa Cruz Biotechnology (3 mentions) Anti caspase 3, by BD (2 mentions) Anti parp 1, by Santa Cruz Biotechnology (2 mentions) Pristimerin, by Merck Group (1 mentions) Caspase 9 antibodies, by BD (1 mentions) Ciap 1, by Santa Cruz Biotechnology (1 mentions) Anti p mtor ser2448, by Cell Signaling Technology (1 mentions) Caspase 8, by BD (1 mentions) Caspase 9, by BD (1 mentions) Anti p akt ser473, by Cell Signaling Technology (1 mentions)

3 protocols using 96 aqueous one solution proliferation assay system

Pristimerin-Induced Apoptosis in Cancer Cells

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Pristimerin was purchased from Sigma Chemicals (St. Louis, MO). Anti-caspase-3 and caspase-9 antibodies were purchased from BD Pharmingen (San Diego, CA). Anti-PARP-1, anti-Bcl-2, anti-Bcl-xL and anti-survivin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). 96 AQueous One Solution Proliferation Assay System was from Promega (Madison, WI). Annexin V-FITC apoptosis detection kit was purchased from BD Pharmingen and mitochondrial potential sensor JC-1 was obtained from Molecular Probes, Invitrogen (San Diego, CA). Caspase-3 activity kit was from Clontech Laboratories (Mountain View, CA). Stock solution of PM (100 mM) was prepared in DMSO and all test concentrations were prepared by diluting stock solution in tissue culture medium.

DEEB D., GAO X., LIU Y.B., PINDOLIA K, & GAUTAM S.C. (2014). Pristimerin, a quinonemethide triterpenoid, induces apoptosis in pancreatic cancer cells through the inhibition of pro-survival Akt/NF-κB/mTOR signaling proteins and anti-apoptotic Bcl-2. International Journal of Oncology, 44(5), 1707-1715.

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Cytotoxicity Evaluation of Phenethyl Isothiocyanate

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PM was purchased from Sigma Chemicals (St. Louis, MO). Anti-PARP-1, anti-Bcl-2, anti-Bcl-xL and anti-survivin antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). The 96 AQueous One Solution Proliferation Assay System was from Promega (Madison, WI). Annexin V-FITC apoptosis detection kit was purchased from BD Pharmingen (San Diego, CA). Stock solution of PM (100 mM) was prepared in DMSO and all test concentrations were prepared by diluting stock solution in tissue culture medium.

LIU Y.B., GAO X., DEEB D., BRIGOLIN C., ZHANG Y., SHAW J., PINDOLIA K, & GAUTAM S.C. (2014). Ubiquitin-proteasomal degradation of antiapoptotic survivin facilitates induction of apoptosis in prostate cancer cells by pristimerin. International Journal of Oncology, 45(4), 1735-1741.

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Pristimerin-Induced Apoptosis Pathway

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Pristimerin (PM) was purchased from Sigma Chemicals (Saint Louis, MO). Anti-caspase-3, caspase-8, and caspase-9 antibodies were purchased from BD Pharmingen (San Diego, CA). Anti-p-Akt (ser⁴⁷³) and anti-p-mTOR (ser²⁴⁴⁸) antibodies were from Cell Signaling Technology (Danvers, MA). Anti-NF-κB (p65), anti-PARP-1, anti-Bcl-2, anti-Bcl-xL, c-IAP1 and anti-survivin antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). 96 AQueous One Solution Proliferation Assay System was from Promega (Madison, WI). Annexin V-FITC apoptosis detection kit was purchased from BD Pharmingen (San Diego, CA) and mitochondrial potential sensor JC-1 was obtained from Molecular Probes, Invitrogen (San Diego, CA).

Gao X., Liu Y., Deeb D., Arbab A.S, & Gautam S.C. (2014). Anticancer activity of pristimerin in ovarian carcinoma cells is mediated through the inhibition of prosurvival Akt/NF-κB/mTOR Signaling. Journal of experimental therapeutics & oncology, 10(4), 275-283.

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