Fibrin clots were prepared on glass slides by dropping 20 µl mixture of fibrinogen (1 mg/ml) and CaCl2 (2 mM) in PBS with or without prion peptide (20 µM), followed by addition of 1 U/ml thrombin to initiate polymerization. After 1 h, jelly-like clots on the slides were gently washed with Milli Q water, allowed to dry, coated with gold using a sputter coater (Quorum Technologies, model SC7620) and examined under a scanning electron microscope (Zeiss, model EVO 18). A secondary electron detector and energy dispersive spectrometer were used for morphological studies and elemental analysis, respectively.
Sc7620
Manufactured by Zeiss
Sourced in Germany
The Zeiss SC7620 is a high-performance scanning electron microscope (SEM) designed for advanced material analysis and characterization. It provides a comprehensive set of tools for imaging, elemental analysis, and precise sample manipulation. The SC7620 delivers exceptional image quality, high resolution, and advanced analytical capabilities to support a wide range of research and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Evo 18, by Zeiss (2 mentions)
Leo 1430, by Zeiss (1 mentions)
Axioplan light microscope, by Zeiss (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Polaron e3000, by Quorum Technologies (1 mentions)
Araldite cy212, by Merck Group (1 mentions)
Geminisem 450, by Zeiss (1 mentions)
Geminisem 300, by Zeiss (1 mentions)
6 protocols using sc7620
1
Fibrin Clot Morphology Analysis
2
Morphological Characterization of EW
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The
morphological features
of the EW were analyzed using a field emission scanning electron microscope.
The lyophilized samples were sputter-coated with gold (Emitech, SC7620)
for FESEM micrograph measurements (Carl Zeiss, Germany). Particle
size, pore size, and their size distribution profiles were determined
from at least 50 measurements on SEM images using image analysis software
(ImageJ 1.50i).
morphological features
of the EW were analyzed using a field emission scanning electron microscope.
The lyophilized samples were sputter-coated with gold (Emitech, SC7620)
for FESEM micrograph measurements (Carl Zeiss, Germany). Particle
size, pore size, and their size distribution profiles were determined
from at least 50 measurements on SEM images using image analysis software
(ImageJ 1.50i).
3
Trachea Tissue Fixation and Decellularization
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Specimens of trachea were fixed by immersion in 4% glutaraldehyde (Merck) in 0.1 M Sorensen phosphate buffer solution (SPBS; pH 7.2) for 4 hours and post-fixed in 1% SPBS buffered osmium tetroxide (OsO4) for 2 hours. Samples were dehydrated in an ascending graded ethanol series and were embedded in Araldite CY212 (Merck). Semithin sections were prepared using an ultramicrotome (Ultratome NOVA-LKB), counterstained with 1% toluidine blue, and examined with a Zeiss Axioplan light microscope (Jena, Germany). To qualitatively evaluate decellularized matrix structure, matrices were fixed with 4% (v/v) glutaraldehyde in a buffered solution of 0.1 M SPBS (pH 7.2). After rinsing in Sorensen phosphate, specimens were dehydrated thr-ough an ethanol gradient, critical point dried (Polaron, E3000; Quorum Technologies, West Sussex, UK), sputter coated (Polaron, SC7620) with gold and analyzed using Zeiss LEO 1430 sca-nning electron microscope (SEM; Oberkochen, Germany).
4
Nanoparticle Characterization via SEM
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The particles were prepared by double emulsion, and the solvent evaporation method was subjected to scanning electron microscopy (SEM) for analysis of size and surface morphology (Carl ZEISS-EVO 18, Germany) using tungsten filament at an operating voltage of 10 kV. Approximately 10 µg of the sample was coated with gold atoms using a sputter coater (SC7620 ‘mini’ sputter coater and glow discharge system, to enhance scattering of electrons) with a vacuum pressure maintained at 1.32 × 10–5 mbar. The sample was loaded into the aluminum holder stub using tweezers and double-sided carbon tape.
5
SEM Analysis of Phytopathogen Morphology
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The SEM study was performed to observe the effects of extracellular secondary metabolites of CNE6 on the cellular morphology of the isolated pathogen CRP1. Mycelia of the phytopathogenic strain CRP1 were taken from the edge of inhibition zones produced in dual culture plates and were considered the treated mycelia. Alternatively, mycelia taken from a freshly grown culture of CRP1 on ME agar plates were regarded as the control. Mycelia were prefixed for 30 min using 2% glutaraldehyde in 20 mM Na-P buffer (pH 6.5) plus 5% dimethyl sulfoxide (DMSO). Then, samples were postfixed for another 30 min using 1% osmium tetroxide dissolved in 50 mM Na-P buffer (pH 6.5). After washing with sterilized distilled water, the mycelia were dehydrated by passage through a series of alcohol grades (30 to 100%) and by retaining them at least 10 min in each grade (47 (link)). The dehydrated mycelia were then gold coated by using an ion sputter (Quorum sputter coater, model SC7620) and observed through the microscope (Zeiss; GeminiSEM 450).
6
Hydrogel Microstructure Analysis
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Hydrogel samples with different components were freeze-dried for 24 h and gold was sprayed on the longitudinal section with Quorum SC7620 sputtering coater for 45 s, followed by observation with a ZEISS GeminiSEM 300 scanning electron microscope at an accelerated voltage of 15 Kv.
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