Generation of the TRP120 wild type and TRP120C520S mutant expression construct (pAcGFP1 and pBAD/Thio) and expression and purification of recombinant TRP120 have been described previously [12 (link),15 (link),29 (link)]. ENO-1 recombinant protein was obtained from a commercial source (Abnova, Taipei, Taiwan). Rabbit or mouse anti-TRP120 antibodies have been described previously. Other antibodies used in this study were anti-FK2 (Enzo), anti-K48 (CST), anti-K63 (CST), anti-UbcH5b (CST), anti-ENO-1 (Abcam), anti-PDH (Santa Cruz) and anti-GAPDH (Proteintech, Rosemont, IL, USA). All antibodies used for immunofluorescence were tested by the vendor to ensure specificity and confirmed by Western immunoblotting, immunofluorescent microscopy or both. ENO-1 (siRNAs) and a control siRNA were obtained from GE Dharmacon (Lafayette, CO, USA).
Anti pdh
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-PDH is a laboratory product that functions as an antibody specific to the Pyruvate Dehydrogenase (PDH) complex. The PDH complex is a crucial enzyme involved in cellular metabolism, catalyzing the conversion of pyruvate to acetyl-CoA. Anti-PDH can be used to detect and quantify the presence of the PDH complex in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti eno1, by Abcam (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Anti hsp70, by Cell Signaling Technology (1 mentions)
Anti ha tag antibody, by Merck Group (1 mentions)
Anti mtch2, by Proteintech (1 mentions)
Anti hsp90, by Cell Signaling Technology (1 mentions)
2 protocols using anti pdh
1
Recombinant TRP120 Protein Expression
2
Antibody Validation for Organelle Proteins
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The antibodies against Act1, Tom70, and Tom22 were raised in rabbits using recombinant purified proteins. The secondary antibody was obtained from Bio-Rad (Goat Anti-Rabbit IgG [H+L]-HRP Conjugate #172-1019) or ImmunoReagents Inc. (Goat anti-Rabbit IgG [H&L]—Affinity Pure, HRP Conjugate, #GtxRb-003-DHRPX, Goat anti-Mouse IgG [H&L]—Affinity Pure, HRP Conjugate, #GtxMu-003-DHRPX). The horseradish-peroxidase coupled HA antibody was purchased from Roche (Anti-HA-Peroxidase, High Affinity [3F10], #12 013 819 001). The HA-tag antibody used in immunofluorescence and Western blot assays was purchased from Sigma-Aldrich (Anti-HA-Tag antibody produced in rabbits, #SAB4300603). The Tom70 antibody was purchased from Sigma-Aldrich (Anti-TOMM70 antibody produced in rabbit, #HPA048020). For analysis of isolated human mitochondria, the following additional antibodies were used: Anti-Hsp90 (#4874S; Cell Signaling Technology), Anti-PDH (#sc-377092; Santa Cruz), Anti-MTCH2 (#16888-1-AP; Proteintech), Anti-Hsp70 (#4872S; Cell Signaling Technology), Anti-GAPDH (#sc-32233; Santa Cruz), Anti-AK2 (rabbit, selfmade). Antibodies were diluted in 5% (wt/vol) nonfat dry milk in 1× TBS buffer in a 1:1,000 dilution, except for: Anti-HA-Peroxidase 1:500, Anti-Rabbit 1:10,000, and Anti-Mouse 1:5,000.
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