Stool specimens were allowed to thaw at room temperature and were diluted 7-fold in buffer with protease inhibitors (RIPA, radioimmunoprecipitation assay buffer). The samples were vortexed, centrifuged and the supernatants were used to measure the biomarkers. The fecal myeloperoxidase (MPO), lactoferrin (FL), lipocalin-2 (Lcn-2) and calprotectin (FC) concentrations were measured using commercially available kits that employed polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) methods, according the manufacturer’s instructions. MPO and Lcn-2 levels were determined using human kits from R&D systems, Inc (Minneapolis, USA). The FL concentration was analyzed using a BD (IBD-SCAN); TechLab Inc. (Blacksburg, USA). The FC concentration was tested using a Calprotectin Elisa Assay Kit, Eagle Biosciences, Inc. (Nashua, USA). Total protein of each sample was assayed using the BCA Protein Assay Kit from Pierce (Pittsburgh, PA). The absorption was measured using an Epoch plate reader, Bio-tek Instruments, Inc. (USA). Units were expressed as ng/mg of stool or as ng/µg protein for protein normalized data.
Human kits
Manufactured by R&D Systems
Sourced in United States
The Human kits from R&D Systems are laboratory products designed for various research applications. They provide the necessary tools and reagents for researchers to conduct experiments and analyses related to human samples or cells. The core function of these kits is to facilitate the preparation, detection, or measurement of specific analytes or targets within human-derived materials, without providing interpretations or recommendations on their intended use.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using human kits
1
Fecal Biomarker Quantification Protocol
2
Fecal Biomarker Quantification Protocol
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!