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Prepacked column

Manufactured by Merck Group
Sourced in United States

Prepacked column is a type of laboratory equipment used for chromatographic separation and purification of various compounds. It consists of a cylindrical column pre-filled with a specific stationary phase material, ready for immediate use. The core function of a prepacked column is to facilitate efficient separation and isolation of target analytes from complex mixtures.

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2 protocols using prepacked column

1

Hydrogel Fabrication from PEGDA Monomers

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Poly(ethylene glycol) diacrylate (PEGDA, MW = 575 Da), N-vinyl pyrrolidone (NVP), potassium monobasic phosphate (phosphate), sodium hexametaphosphate (polyphosphate, (NaPO3)n MW = 101.9618*n)), potassium persulfate (KPS), sorbitan monooleate (SPAN80), PEG (20) sorbitan monolaurate (TWEEN20), cyclohexane, acetone, sodium citrate, ammonium sulfate, magnesium sulfate and tryptic soy broth (TSB) were purchased from Sigma-Aldrich. Agar was purchased from Fisher Scientific. Tryptone was purchased from EMD Millipore. Monomethyl ether hydroquinone (MEHQ) inhibitor (400–600 ppm) was removed from the PEGDA monomer by running the stock solution through a prepacked column (Sigma Aldrich). Sodium hydroxide inhibitor was removed from the NVP monomer by passing the stock solution through a 0.2 µm syringe filter. All other chemicals were used as received.
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2

Hydrogel Synthesis and Purification

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Poly(ethylene glycol) diacrylate (PEGDA, MW = 575 Da), N-vinyl pyrrolidone (NVP), potassium phosphate monobasic (phosphate, Pi), sodium hexametaphosphate (polyphosphate, PPi), potassium persulfate (KPS), sorbitan monooleate (SPAN80), PEG (20) sorbitan monolaurate (TWEEN20), cyclohexane, and acetone were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ultra-high purity (UHP) nitrogen was purchased from Airgas (Radnor, PA, USA).
Monomethyl ether hydroquinone (MEHQ) inhibitor (400–600 ppm) was removed from the PEGDA monomer by passing the solution through a prepacked column (Sigma Aldrich). Sodium hydroxide inhibitor was removed from the NVP monomer by passing the solution through a 0.2 μm syringe filter (Millipore, Burlington, MA, USA). All other materials were used as received.
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