Hemin 51 280

CD spectra: All oligonucleotides were purchased from Sigma-Aldrich Co. LLC. (Japan) without further purification. Oligonucleotides for CD spectra were prepared in Li + solution (20 mM lithium cacodylate, pH 7.0, 100 mM LiCl), Na + solution (20 mM lithium cacodylate, pH 7.0, 100mM NaCl) or K + solution (20 mM lithium cacodylate, pH 7.0, 100 mM KCl). Annealing was performed by heating to 95°C for 5 min and cooling down to room temperature slowly. CD spectra were measured in 0.5-nm steps from 340 to 220 nm using JASCO J-805LST Spectrometer in a 1-cm quartz cuvette.
UV melting experiment: UV-Vis melting temperature analyses were performed on a spectophotometer V-650 (JASCO, Japan) with a thermocontrolled PAC-743R cell changer (JASCO, Japan) and a refrigerated and heating circulator F25-ED (Julabo). Hemin (51280) and TMPyP4 (323497) were purchased from Sigma-Aldrich Co. LLC. (Japan). Oligonucleotides (5 µM) in K + solution (20 mM lithium cacodylate, pH 7.0, 5 mM KCl) and 1 eq compounds were added. Annealing was performed by heating to 95°C for 5 min and cooling down to room temperature slowly. Melting temperature(Tm of each samples were measured at 295 nm from 20 to 90°C at a rate of 1.0 °C /min. ΔTm were calculated by the Tm values of samples with and without compounds.