Log In Sign up
The largest database of trusted experimental protocols

Anti phosphotyrosine antibody clone 4g10 agarose conjugate

Manufactured by Merck Group
Visit Supplier

The Anti-phosphotyrosine antibody (clone 4G10) agarose conjugate is a laboratory product used for the detection and purification of phosphorylated tyrosine-containing proteins. The antibody is covalently coupled to agarose beads, providing a convenient tool for immunoaffinity chromatography and immunoprecipitation applications.

Automatically generated - may contain errors

Lab products found in correlation

Vinculin, by Merck Group (2 mentions) Her2 and her3, by Santa Cruz Biotechnology (2 mentions) Immunofluorescently labeled antibody to mouse igg, by Rockland Immunochemicals (2 mentions) Antibody to rabbit igg, by Thermo Fisher Scientific (2 mentions) Mk2206, by MedChemExpress (2 mentions) P mek, by Cell Signaling Technology (2 mentions) Odyssey scanner, by LI COR (2 mentions) Ripa buffer, by Merck Group (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Phosphatase inhibitor, by Roche (2 mentions) Gefitinib, by MedChemExpress (2 mentions)

Close spelling variants of this product

Agarose (759 citations) Anti-phosphotyrosine 4G10 (36 citations) Anti-phosphotyrosine antibody (4G10, (34 citations) Anti-phosphotyrosine (27 citations) Phosphotyrosine (4G10; (16 citations) Anti-phosphotyrosine antibody (clone 4G10, (15 citations) Clone 4G10 (11 citations) Anti-phosphotyrosine antibody (8 citations) Anti-phosphotyrosine clone 4G10 (6 citations)

2 protocols using anti phosphotyrosine antibody clone 4g10 agarose conjugate

1

Molecular Signaling Analysis in Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors were homogenized in ice-cold RIPA buffer (Sigma) containing protease inhibitor cocktail (Roche) and phosphatase inhibitor (Roche). For tumor lysates, standard western blotting was conducted as described 15 using antibodies against p-AKT (Ser473), p-AKT(Thr308), p-FoxO1 (Thr24)/FoxO3a(Thr32), p-ERK(1/2) (Thr202/Tyr 204), ERK, pMEK(Ser217/Ser221), MEK (Cell Signaling Technology), Her2 and Her3 (Santa Cruz Technology) and vinculin (Sigma-Aldrich). For immunoprecipitation assay, anti-phosphotyrosine antibody (clone 4G10) agarose conjugate (EMD Millipore) was used. Immunofluorescently labeled antibody to mouse IgG (Rockland Immunochemicals) and antibody to rabbit IgG (Molecular Probes) were used to visualize western blots on an Odyssey scanner (Li-Cor). MK2206 and Gefitinib were purchased from MedChemexpress and Selleck, respectively.
Cheng H., Liu P., Ohlson C., Xu E., Symonds L., Isabella A., Muller W.J., Lin N.U., Krop I.E., Roberts T.M., Winer E.P., Arteaga C.L, & Zhao J.J. (2015). PIK3CAH1047R and Her2 initiated mammary tumors escape PI3K dependency by compensatory activation of MEK-ERK signaling. Oncogene, 35(23), 2961-2970.
+ Open protocol
+ Expand
2

Molecular Signaling Analysis in Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumors were homogenized in ice-cold RIPA buffer (Sigma) containing protease inhibitor cocktail (Roche) and phosphatase inhibitor (Roche). For tumor lysates, standard western blotting was conducted as described 15 using antibodies against p-AKT (Ser473), p-AKT(Thr308), p-FoxO1 (Thr24)/FoxO3a(Thr32), p-ERK(1/2) (Thr202/Tyr 204), ERK, pMEK(Ser217/Ser221), MEK (Cell Signaling Technology), Her2 and Her3 (Santa Cruz Technology) and vinculin (Sigma-Aldrich). For immunoprecipitation assay, anti-phosphotyrosine antibody (clone 4G10) agarose conjugate (EMD Millipore) was used. Immunofluorescently labeled antibody to mouse IgG (Rockland Immunochemicals) and antibody to rabbit IgG (Molecular Probes) were used to visualize western blots on an Odyssey scanner (Li-Cor). MK2206 and Gefitinib were purchased from MedChemexpress and Selleck, respectively.
Cheng H., Liu P., Ohlson C., Xu E., Symonds L., Isabella A., Muller W.J., Lin N.U., Krop I.E., Roberts T.M., Winer E.P., Arteaga C.L, & Zhao J.J. (2015). PIK3CAH1047R and Her2 initiated mammary tumors escape PI3K dependency by compensatory activation of MEK-ERK signaling. Oncogene, 35(23), 2961-2970.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!