Reagent grade solvents

Manufactured by Thermo Fisher Scientific

Sourced in United States

Reagent grade solvents are high-purity chemical compounds used in various laboratory applications. They are designed to meet strict standards for analytical and scientific purposes. These solvents are typically used as reference materials, as components in reagent formulations, or for sample preparation and extraction processes.

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Lab products found in correlation

4 protocols using reagent grade solvents

Isotopic Labeling of Biomolecules

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Triethyl orthoformate, ethyl cyanoacetate, diethyl malonate, sodium metal, lithium aluminum hydride solution, triethylamine, potassium persulfate, silver nitrate, and ¹⁵N₂-urea were obtained from Sigma-Aldrich (St. Louis, MO). Potassium perruthenate, paraformaldehyde, N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide + 1% tert-butyldimetheylchlorosilane (MTBSTFA + 1% TBDMCS), reagent grade solvents, and anhydrous solvents were purchased from Fisher Scientific (Pittsburgh, PA). ¹⁵N₂-Orotic acid, ²H₄-thymine, DMSO-d₆, and methanol-d₄ were purchased from Cambridge Isotope Laboratory (Cambridge, MA). ²H₄-5-methylcytosine and ²H₂-cytosine were obtained from CDN Isotopes (Quebec, Canada). ¹⁵N₂-uracil was purchased from Euriso-top (Saint-Aubin, France).

Patel J.P., Sowers M.L., Herring J.L., Theruvathu J.A., Emmett M.R., Hawkins B.E., Zhang K., DeWitt D.S., Prough D.S, & Sowers L.C. (2015). Measurement of Postreplicative DNA Metabolism and Damage in the Rodent Brain. Chemical research in toxicology, 28(12), 2352-2363.

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Synthesis of Functionalized Polymers

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Chemicals including ε-caprolactone, diethyl fumarate, propylene glycol, tin(II) 2-ethylhexanoate (Sn(Oct)₂), (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethanol (BCN-OH), oxalyl chloride, POSS®-Octahydroxypropyldimethylsilyl (POSS-OH), sodium azide (NaN₃), were purchased from Sigma Aldrich Co. (Milwaukee, WI) and used as received. Reagent grade solvents were purchased from Fisher (Pittsburgh, PA). Dichloromethane was purchased from Fisher and distilled with calcium hydride (CaH₂) to obtain anhydrous CH₂Cl₂. Potassium carbonate (K₂CO₃) obtained from Sigma Aldrich Co. was dried in a 100°C oven to exclude moistures. Unless noted otherwise, the reagents or chemicals in the study were purchased from Fisher or Sigma.

Liu X., Camilleri E.T., Li L., Gaihre B., Rezaei A., Park S., Miller AL I.I., Tilton M., Waletzki B.E., Terzic A., Elder B.D., Yaszemski M.J, & Lu L. (2021). Injectable catalyst-free “click” organic-inorganic nanohybrid (click-ON) cement for minimally invasive in vivo bone repair. Biomaterials, 276, 121014.

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Purification of Monomers for Copolymerization

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n-BA (≥99% purity), VAc (≥99% purity) and azobisisobutyronitrile (AIBN, 98% purity) were purchased from Sigma Aldrich (Oakville, ON, Canada). MDO was obtained from Wacker Chemie (München, Germany) and was used as received. Deuterated chloroform (Fisher (Ottawa, ON, Canada), 99.8%) was used for characterization. Reagent grade solvents (Fisher) for sample workup (e.g., acetone, methanol) were used as packaged. BA and VAc monomers underwent a rigorous purification process, passing through inhibitor removal columns acquired from Sigma Aldrich. This process effectively eliminated any traces of inhibitors, such as hydroquinone or monomethyl ether hydroquinone.

Movafagh M., Meek K.M., Scott A.J., Penlidis A, & Dubé M.A. (2024). Bulk Free Radical Terpolymerization of Butyl Acrylate, 2-Methylene-1,3-Dioxepane and Vinyl Acetate: Terpolymer Reactivity Ratio Estimation. Polymers, 16(10), 1330.

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Orthodontic Acrylic Resin Synthesis and Characterization

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An auto-polymerizing orthodontic acrylic resin was purchased from Ortho-Jet, Lang Dental Manufacturing Co. Inc. (Chicago, Ill, USA). Thiazolyl blue tetrazolium bromide, isoamyl acetate, 2,2′-azobisisobutyronitrile (AIBN) and 2-methoxyethyl acrylate (MEA) were purchased from Sigma-Aldrich (St Louis, MO, USA). Methyl mercaptopropionate (MMP) and reagent-grade solvents were purchased from Fisher Scientific (Waltham, MA, USA). Methacryloyl thiocarbamoyl Rhodamine-B (RhB) was purchased from Polysciences (Washington, PA, USA). Prior to polymerization, MEA monomer was passed through an alumina column to remove the inhibitor. AIBN was recrystallized with hot methanol, prior to use. Other chemicals were used as received without further purification. PBS was purchased from Welgene (Gyeongsan, Gyeongsangbuk-do, Korea). BSA and micro-bicinchoninic acid (Micro BCATM Protein Assay Kit) were purchased from Pierce Biotechnology (Rockford, IL, USA). Dulbecco's modified Eagle's medium was obtained from Gibco BRL (Gaithersburg, MD, USA) and used as a cell culture medium. LIVE/DEAD bacterial viability kits were purchased from Molecular Probes (Eugene, OR, USA).

Jin J., Bhat R., Mangal U., Seo J.Y., Min Y., Yu J., Kim D.E., Kuroda K., Kwon J.S, & Choi S.H. (2022). Molecular weight tuning optimizes poly(2-methoxyethyl acrylate) dispersion to enhance the aging resistance and anti-fouling behavior of denture base resin. Biomaterials science, 10(9).

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