Gibcopbs tablets

Manufactured by Thermo Fisher Scientific

GibcoPBS tablets are a sterile, endotoxin-tested, ready-to-use phosphate buffered saline solution. They are designed to maintain physiological pH and osmolarity, and are commonly used as a buffer in various laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Methanol, by Avantor (1 mentions) Dope peg2000, by Avanti Polar Lipids (1 mentions) Deuterated water d2o, by Merck Group (1 mentions) Pd miditrap column, by GE Healthcare (1 mentions)

2 protocols using gibcopbs tablets

Lipid Preparation for Biophysical Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

DPPC, POPC, DOPC, and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene
glycol)-2000] (DOPE-PEG₂₀₀₀) were purchased from Avanti
Polar Lipids (Alabaster, AL). Methanol (VWR Chemicals) and diisopropyl
ether (VWR Chemicals) were used as purchased. Deuterated water (D₂O) was purchased from Sigma-Aldrich. PBS in deuterated water
(dPBS) was prepared by dissolving the appropriate amount of Gibco
PBS tablets (manufacturer’s composition: 10 mM sodium phosphates,
2.68 mM potassium chloride, 140 mM sodium chloride) (ThermoFisher
Scientific) in D₂O.

Nele V., Holme M.N., Kauscher U., Thomas M.R., Doutch J.J, & Stevens M.M. (2019). Effect of Formulation Method, Lipid Composition, and PEGylation on Vesicle Lamellarity: A Small-Angle Neutron Scattering Study. Langmuir, 35(18), 6064-6074.

+ Open protocol

+ Expand

SANS Analysis of Polymersomes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Empty polymersomes and polymersomes
loaded with 2 mM Et and COOMe were prepared,
as described previously. Samples then underwent buffer exchange to
deuterated PBS for SANS measurements (Gibco PBS tablets (ThermoFisher
Scientific) dissolved in D₂O). Samples in PBS were passed
through a PD MidiTrap column (GE Healthcare) equilibrated in deuterated
PBS. All of the measurements were performed at the ZOOM beamline of
the ISIS Pulsed Neutron Source at the Rutherford Appleton Laboratory,
Didcot, UK. A sample changer and 2 mm path length quartz cuvette cells
were used. The beamline was configured with L1 = L2 = 4 m, where L1
is the source to sample distance and L2 is the sample to detector
distance, yielding a scattering variable (Q) range
of 0.004 to 1 Å^–1. Samples were measured at
15 μAmps (SANS) and 5 μAmps (TRANS) at 25 °C.
SANS data were reduced with MantidPlot.^{53 (link)} SasView v5.0.4. (http://www.sasview.org/) was employed to fit the experimental data over a q range of 0.01 < q < 0.1 Å^–1 with a polydispersity of 0.1 on the radius and shell thickness.
Scattering length density (sld) of core, shell, and solvent were set
to to 6.3, 4, and 6.3 × 10^–6/Å², respectively.

Saunders C., Foote J.E., Wojciechowski J.P., Cammack A., Pedersen S.V., Doutch J.J., Barriga H.M., Holme M.N., Penders J., Chami M., Najer A, & Stevens M.M. (2023). Revealing Population Heterogeneity in Vesicle-Based Nanomedicines Using Automated, Single Particle Raman Analysis. ACS Nano, 17(12), 11713-11728.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!