Dp controller and manager software

Periodic Acid-Schiff (PAS) staining was performed in the formalin-fixed/paraffin-embedded lung tissues. Tissue sections were examined with an Olympus BX40 microscope in conjunction with an Olympus U-TV0.63XC digital camera (Olympus Corp., Melvile, NY). Images were acquired using DP Controller and Manager software (Olympus Corp.).

Differentiated cell cultures were fixed with 10% normal-buffer formalin, and embedded in paraffin (University of Wisconsin Histology Lab, Madison, WI). Five μm sections were adhered to slides which were deparaffinized and then rehydrated. For antigen retrieval, slides were incubated with proteinase K (40 μg/mL in PBS, 10 min, 37 °C). Peroxidases were blocked (5 min, RT) with Peroxidazed 1 (Biocare Medical, Concord, CA). Slides were blocked (3% FBS, 2% goat serum, 0.2% Tween-20, 1.25% Human BD Fc Block™, 1 h, RT), incubated (1:200 in blocking buffer, 2 h, RT) with anti-C15-VP2 mouse monoclonal antibody (kindly provided by MedImmune Inc., Gaithersberg MD), Mach 4 Universal Probe and then Polymer (15 min, RT each, Biocare Medical, Concord, CA), Betazoid DAB (5 min, RT, Biocare Medical, Concord, CA), and counterstained with CAT hematoxylin or eosin (30s, RT, Biocare Medical, Concord, CA). Images from labeled slides were acquired and analyzed using an Olympus BX60 light microscope with DP Controller and Manager software (Shinjuku-ku, Tokyo, Japan).