RNAi-PPARG MCF-7 cells, Scramble-PPARG MCF-7 cells and wild-type MCF-7 cells, 8 × 105 in each case, were seeded on 60-mm culture plates and incubated at 37 °C and 5 % CO2. After 24 h, the attached cells were scratched three times in parallel with a 1000-μl pipette tip (~377 μm) and treated with 10 μM 6IL or vehicle (ethanol) for 24 h. After this time, three new parallel wounds were performed as width control; the number of migrating cells was obtained by counting the number of cells inside this width control. The count was measured by Leica Application Suite Version 2.8.1., and the images were captured with a 10× objective lens using a Leica DM 2500 microscope and DFC 420 camera (Leica Microsystems, CMS GmbH, Germany). The results are presented as number of migrating cells of treated (6IL) vs. control (Ctrl) groups (mean ± SD). At least three fields were analyzed for each plate, and each group was analyzed in three independent experiments.
Application suite version 2.8.1
Manufactured by Leica
Sourced in Germany
Leica Application Suite Version 2.8.1 is a software application that provides a user interface for controlling and managing various Leica laboratory equipment. The core function of the software is to enable efficient operation and data management of Leica instruments.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using application suite version 2.8.1
1
RNAi PPARG Modulates Cell Migration
2
Immunohistochemical Analysis of Ikaros Expression
3
Tissue Fixation and Sectioning Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Animals were prefixed in 2.5% glutaraldehyde with 0.05% OsO4 for 10 min on ice, fixed in 2.5% glutaraldehyde in cacodylate buffer for 1 h at 4 C and post-fixed for 1 h in 1% OsO4 at 4 C (for a detailed protocol, see Salvenmoser et al., 2010) . After dehydration in a graded ethanol series, the specimens were embedded in Spurr's resin. Sections were cut with a Leica ultracut UCT microtome. Semi-thin sections were stained with methylene blue and AZUR II according to Richardson et al. (1960) and examined with a Leica DM5000B light microscope. Images were taken with a Leica DFC camera using Leica Application Suite version 2.8.1. Thin sections were contrasted with uranyl acetate and lead citrate and examined with a Zeiss Libra 120 energy filter transmission electron microscope. Images were made with the iTEM software (Olympus) and a TRS 2048 high speed digital camera (Tr€ ondle, Germany).
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