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5 protocols using hcl acid

1

HPLC Analysis of Ginkgo Leaf Extracts

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In the current work, all chemical substances used were analytical grade. For HPLC analysis, we used methanol (purity 99.9%) from Merck, UK, H3PO4 acid, purity 85%, NaH2PO4, HCl acid, purity 37%, and NaOH (Scharlau, Spain). The buffer solution was prepared by weighing about 16.8 g of NaH2PO4 and 0.5 mL of H3PO4 acid 85% in 700 mL of deionized water. Ginkgo leaves were collected from a tree growing in Wuhu, Anhui, China, in the beginning of the summer season in 2020. The species was authenticated at Al-Azhar University, Assuit, Egypt (Botany and Microbiology Department, Faculty of Science).
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2

Zircon Raffinate Solution Processing

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The Egyptian Nuclear Materials Authority provides zircon raffinate solution. HCl acid, NaOH, HNO3 acid, and H2SO4 acid were purchased from Merck, Germany. Acrylic acid (AA) with 99% purity was obtained from Elf Chem. Co, France. Loba Chemie India provided 99% pure malic acid (MA). Methylene bis-acrylamide (DAM) and extra-pure graphite powders were provided by Merck, Germany. BDH Chemicals, Ltd., England, produced potassium permanganate (KMnO4) and sodium nitrate (NaNO3). Alpha Co., India, also delivered hydrogen peroxide, while ascorbic acid was obtained from Bratachem Co., Egypt.
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3

Extraction and Characterization of Inulin and Quercetin

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Organic inulin from Agave (6179) powder (Microingredients®, Montclair, CA, USA). In addition, quercetin 95% (HPLC, Sigma Aldrich, San Luis, MO, USA), Absolute ethanol 99.5% (Sigma Aldrich, San Luis, MO, USA), Milli Q (Milli-Q Integral 15 Equipment, Merck México, Estado de Mexico, Mexico), distilled water (Milli-Q Integral 15 Equipment, Merck México, Estado de Mexico, Mexico), Acetonitrile 99.95% (DEQ), Methanol 99.96% (J.T. Baker), Acetone 99.95% (Sigma Aldrich, San Luis, MO, USA), HCl acid 37.40% (Sigma Aldrich, San Luis, MO, USA), NaOH 99.95% (Sigma Aldrich, San Luis, MO, USA) and glacial acetic acid 99.96% (Sigma Aldrich, San Luis, MO, USA) were obtained.
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4

Biogenic Synthesis of Iron Oxide Nanoparticles

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Methyl green dye, NaCl salt, and HCl acid were purchased from Sigma-Aldrich. JUN-Fe3O4, ROS-Fe3O4, MAT-Fe3O4 and ARM-Fe3O4 NP powders were greenly synthesised using an iron salt (FeCl3·6H2O) (purchased from Biochem Chemopharma Co, Canada) as the precursor and Artemisia herba-alba (L) (Asteraceae family), Matricaria pubescens (L) (Asteraceae family), Juniperus phoenicia (L) (Cupressaceae family), Rosmarinus officinalis (L) (Lamiaceae family) plants as reducing agents. Fe3O4 samples were obtained after 4 months of storage of the synthesized iron oxides in ambient conditions. The freshly synthesized samples were both wüstite and magnetite.67 (link) The chemical structure of MG is presented in Fig. 1.
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5

Greenly Synthesized Magnetic Nanoparticles

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Evans blue, methyl orange dyes, NaCl salt, HCl acid, and NaOH base are purchased from Sigma-Aldrich. JUN–Fe3O4, MAT–Fe3O4, ROS–Fe3O4 and ARM–Fe3O4 nanoparticle powders were greenly synthesised using iron salt (FeCl3·6H2O) (purchased from Biochem Chemopharma Co, Canada) as precursor and Artemisia herba-alba (L) (Asteraceae family), Matricaria pubescens (L) (Asteraceae family), Juniperus phoenicea (L) (Cupressaceae family), and Rosmarinus officinalis (L) (Lamiaceae family) plants as reducing agents. Magnetite samples were obtained after 4 months of storage of the synthesized iron oxides under ambient conditions. Characterization of the freshly synthesized iron oxides samples is presented in Ahmouda et al.38 (link)
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