Rat brain was examined using a 3.0-Tesla MRI animal scanner (Siemens, Germany) which was implemented using a TIM system. For signal excitation and detection, the head of a rat was placed in a custom device with a 30 mm inner diameter. The parameters of the MRI were set as follows: 92 ms TE, 3620 ms TR, 8×8 cm FOV, 256×256 M, 2 NA, 2 mm thickness and 0 mm gap. The head of a rat was placed in a flat skull position and the rat brain was displayed in the center of the image slices. By calculating the “mean density value”, hemisphere intensity was examined using the Image-Pro Plus 5.0 software (Media Cybernetics, USA) after the optimal contrast adjustment. MRI examinations were carried out 24 hours after the MCAO surgery.
3.0 tesla mri animal scanner
Manufactured by Siemens
Sourced in Germany
The 3.0-Tesla MRI animal scanner is a medical imaging device designed for use in veterinary and research settings. It utilizes a powerful 3.0-Tesla magnetic field to generate high-resolution images of animal subjects. The scanner is capable of producing detailed visualizations of anatomical structures and physiological processes within the animal body.
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Lab products found in correlation
2 protocols using 3.0 tesla mri animal scanner
1
Rat Brain MRI After MCAO Surgery
2
T2-Weighted MRI of Rat Hippocampus
Check if the same lab product or an alternative is used in the 5 most similar protocols
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MRI (T2 weighted image, T2WI) was performed as reported by others25 (link). In this study, the hippocampus is the region of interest (ROI) due to its higher sensitivity to hypoxia or edema than other brain regions following FES.
At 12 h following FES, the anesthetized rat was placed on a special frame in supine position, and introduced into a 3.0-Tesla MRI animal scanner (Siemens, Munich, Germany). The head of the rat was set in a “birdcage coil” (diameter = 30 mm). A T1-weighted image sequence was conducted for orientation of subsequent scans. A muscle relaxant was applied continuously via the femoral artery to minimize movement artifacts (diluted atracurium besilate, initial bolus 4 mg/kg followed by 4.5 mg/h; Glaxo Wellcome, Brentford, UK).
T2WI was performed using the following parameters: repetition time (TR) = 2500 ms; echo time (TE) = 8.1–65.6 ms; flip angle = 90°; matrix = 0.63 mm × 0.63 mm; slice thickness = 2 mm; number of averages = 1; echo train length 1. The data sets of T2WI consisted of eight consecutive, 2-mm thick slices without slice gap. All process was conducted by two experienced, independent radiologists. The T2 values of hippocampus each group were recorded and analyzed.
At 12 h following FES, the anesthetized rat was placed on a special frame in supine position, and introduced into a 3.0-Tesla MRI animal scanner (Siemens, Munich, Germany). The head of the rat was set in a “birdcage coil” (diameter = 30 mm). A T1-weighted image sequence was conducted for orientation of subsequent scans. A muscle relaxant was applied continuously via the femoral artery to minimize movement artifacts (diluted atracurium besilate, initial bolus 4 mg/kg followed by 4.5 mg/h; Glaxo Wellcome, Brentford, UK).
T2WI was performed using the following parameters: repetition time (TR) = 2500 ms; echo time (TE) = 8.1–65.6 ms; flip angle = 90°; matrix = 0.63 mm × 0.63 mm; slice thickness = 2 mm; number of averages = 1; echo train length 1. The data sets of T2WI consisted of eight consecutive, 2-mm thick slices without slice gap. All process was conducted by two experienced, independent radiologists. The T2 values of hippocampus each group were recorded and analyzed.
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