Asp718

Manufactured by Roche

Asp718 is a laboratory instrument designed for automated nucleic acid extraction. It utilizes a magnetic bead-based technology to efficiently isolate and purify DNA, RNA, or other nucleic acids from a variety of sample types. The core function of Asp718 is to provide a reliable and consistent method for nucleic acid extraction, enabling downstream analysis and applications.

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Lab products found in correlation

Mmessage mmachine sp6 kit, by Thermo Fisher Scientific (1 mentions) Hindiii, by Roche (1 mentions) Hybond xl, by GE Healthcare (1 mentions) Curix 60, by AGFA HealthCare (1 mentions) Takara la taq, by Takara Bio (1 mentions) Pcdna3 plasmid vector, by Thermo Fisher Scientific (1 mentions)

3 protocols using asp718

Linearized cDNA Transcription

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All mutants and wild-type cDNAs were linearized by Asp718 (Roche). In vitro transcription was performed with SP6 mMESSAGE mMACHINE Kit (AM1340, Thermo Fisher Scientific, Waltham, MA, USA).

Yoon J., Cachau R., David V.A., Thompson M., Jung W., Jee S.H., Daar I.O., Winkler C.A, & Cho S.K. (2021). Characterization of a Compound Heterozygous SLC2A9 Mutation That Causes Hypouricemia. Biomedicines, 9(9), 1172.

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Quantitative Analysis of Retroviral Integrations

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To detect full-length RTM integration, genomic DNA was extracted and digested with HindIII (Roche), which flanks the RTM, resulting in a 4.4 kb DNA fragment, using an RTM specific probe. For evaluation of the VCN genomic DNA was digested with Asp718 (Roche), resulting in a 7 kb fragment, detectable using a GFP specific probe. Digested DNA was separated on a 0.8% agarose gel, transferred to a nylon membrane (Hybond XL, GE Healthcare) and either probed with the ³²P-radiolabeled sequence of a fragment of the RTM (Exon65- exon70- spacer) to detect specific (4.4 kb) and non-specific, rearranged integrations or probed with the ³²P-radiolabeled sequence of a fragment of GFP to detect the number of integration events. To detect the radiolabel signal membranes were exposed to phosphor screens and visualized using Phosphorimaging (Bio-Rad Molecular Imager FX) and they were exposed in an automatic reveal machine Curix60 (AGFA).

Peking P., Koller U., Duarte B., Murillas R., Wolf S., Maetzig T., Rothe M., Kocher T., García M., Brachtl G., Schambach A., Larcher F., Reichelt J., Bauer J.W, & Murauer E.M. (2017). An RNA-targeted therapy for dystrophic epidermolysis bullosa. Nucleic Acids Research, 45(17), 10259-10269.

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Generation of Mouse Inpp4a cDNA Variants

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Mouse Inpp4a cDNAs with C-terminal FLAG tag were generated by PCR using mouse cerebellum cDNAs (wild-type, Inpp4a^ΔEx1,2 KO and Inpp4a^ΔEx23 KO mice) as templates. The primers were 5′-GGGGTACCCCCCACGTGGTCCAAAAGCAAG-3′ (sense), 5′-ATAAGAATGCGGCCGCAAGCTTTCACTTGTCATCGTCATCCTTGTAGTCTGTCTCAACTTTTCCGTAAGTCCCT-3′ (antisense for Inpp4a WT, Δ2, Δ23) and 5′-ATAAGAATGCGGCCGCAAGCTTTCACTTGTCATCGTCATCCTTGTAGTCCGGGCACTTTTGTCTGCCTC-3′ (antisense for Inpp4a CB). TaKaRa LA Taq (TaKaRa Bio.) was used for the PCR reactions. The PCR products containing the full-length Inpp4a cDNAs with FLAG tag were cut with Asp718 (Roche) and NotI (Nippon Gene) and then subcloned into pcDNA3 plasmid vector (Invitrogen). The produced plasmids are referred to as pcDNA3-mouse Inpp4a-FLAG, pcDNA3-mouse Inpp4a ΔEx1,2-FLAG, pcDNA3-mouse Inpp4a ΔEx23, and pcDNA3-mouse Inpp4a CB-FLAG plasmids. Sequencing was performed on both strands.

Tran D.M., Yoshioka N., Bizen N., Mori-Ochiai Y., Yano M., Yanai S., Hasegawa J., Miyashita S., Hoshino M., Sasaki J., Sasaki T, & Takebayashi H. (2023). Attenuated cerebellar phenotypes in Inpp4a truncation mutants with preserved phosphatase activity. Disease Models & Mechanisms, 16(7), dmm050169.

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