The physicochemical analyses of the isolated natural products were essentially performed as previously described [28 (link)]. Optical rotations were measured on a JASCO digital polarimeter (model DIP-3600, JASCO, Tokyo, Japan). UV spectra were determined on a Spectronic Unicam spectrophotometer (Thermo Scientific, Waltham, MA, USA). IR spectra were determined on a JASCO Fourier transform IR-420 spectrometer (JASCO, Tokyo, Japan). 1H and 13C NMR spectra were run on a Bruker spectrometer (Bruker Corporation, Brussels, Belgium) equipped with 5 mm 1H and 13C probes operating at 500 and 125 MHz, respectively, with Tetramethylsilane (TMS) as internal standard. Silica gel 230–400 mesh (Merck, Bielefeld, Germany) and silica gel 70–230 mesh (Merck) were used for flash and column chromatography while precoated aluminum-backed silica gel 60 F254 sheets were used for TLC. Spots were visualized under UV light (254 and 365 nm) or using MeOH–H2SO4 reagent.
Fourier transform ir 420 spectrometer
Manufactured by Jasco
Sourced in Japan
The JASCO Fourier transform IR-420 spectrometer is a laboratory instrument used for the analysis of molecular structures through infrared spectroscopy. It operates by passing infrared radiation through a sample and measuring the absorption or transmittance of the radiation at different wavelengths. The spectrometer utilizes Fourier transform technology to rapidly acquire and process the infrared data, providing detailed information about the chemical composition and molecular structure of the sample.
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Lab products found in correlation
2 protocols using fourier transform ir 420 spectrometer
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Physicochemical Analyses of Isolated Natural Products
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Analytical Techniques for Compound Characterization
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General IR spectra were determined on a JASCO Fourier transform IR-420 spectrometer (JASCO). Ultraviolet spectra were recorded in MeOH on a Hitachi UV 3200 spectrophotometer and infrared spectra on a JASCO 302-A spectrophotometer (Thermo Scientific, Waltham, Chemical shifts are reported in δ (ppm) using tetramethylsilane (TMS) (Sigma-Aldrich, Munich, Germany) as internal standard, while coupling constants (J) were measured in Hz. Column chromatography was carried out on silica gel 230-400 mesh (Merck, Darmstadt, Germany), silica gel 70-230 mesh (Merck, Darmstadt, Germany), and Sephadex LH-20 (Sigma-Aldrich, Munich, Germany). Thin layer chromatography (TLC) was performed on Merck precoated silica gel 60 F254 aluminum foil (Merck, Darmstadt, Germany), and spots were detected using diluted sulfuric acid spray reagent after heating. The molecular composition of the isolated compounds was identified by exact mass determination. All reagents used were of analytical grade.
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