Fvb tg gad gfp 4570swn j mice

Entire procedures were approved by Institutional Animal Care and Use Committee in Anhui, China. The cortical slices (400 μm) were made from FVB-Tg(Gad GFP)4570Swn/J mice (Jackson Lab, Bar Harbor, ME 04609, USA) in postnatal day 22–25. These mice were anesthetized by inhaling isoflurane and decapitated by guillotine. The cortical slices in coronal direction were cut with Vibratome in the oxygenated (95% O₂/5% CO₂) artificial cerebrospinal fluid (ACSF) in concentrations (mM) of 124 NaCl, 3 KCl, 1.2 NaH₂PO₄, 26 NaHCO₃, 0.5 CaCl₂, 4 MgSO₄, 20 dextrose, and 5 HEPES (pH 7.35 at 4°C). These slices were held in the oxygenized ACSF (124 NaCl, 3 KCl, 1.2 NaH₂PO₄, 26 NaHCO₃, 2.4 CaCl₂, 1.3 MgSO₄, 10 dextrose and 5 HEPES with pH 7.35) at 25°C for 2 hours. Each slice was then transferred to a submersion chamber (Warner RC-26G) that was perfused by the oxygenated ACSF at 31°C for whole-cell recording [47 (link),48 (link)]. Chemical reagents were purchased from Sigma.
GABAergic neurons for whole-cell recordings were selected in layer II~III of the sensory cortices based on GFP-labeled neurons under the DIC-fluorescent microscope (Nikon, FN-E600, Japan), in which an excitation wavelength was 488 nm. The neurons demonstrated fast spiking and less adaptation in spike amplitudes and frequencies, the typical properties for interneurons [49 (link)–52 (link)].

Male FVB-Tg(GadGFP)4570Swn/J mice (i.e., GIN mice; The Jackson Laboratory) age six to eight weeks old, weighing 23–28 g, or male CD-1 mice (Harlan) age six to eight weeks old, weighing 30–35 g, were housed in a normal 14/10 h light/dark cycle. Mice were housed in the University of Kentucky vivarium for a minimum of 7 d before experimentation; food and water was provided ad libitum. GIN mice express eGFP in the somatostatinergic subset of GABAergic interneurons in cortical brain regions; in the hilus, somatostatin is expressed in ∼95% of eGFP-labeled (eGFP+) hilar interneurons (Oliva et al., 2000 (link)). All procedures were approved by the University of Kentucky Animal Care and Use Committee and followed the National Institutes of Health guidelines for the care and treatment of animals.