μbondasphere 5 μm c18 column

Example 67

The corresponding oligonucleoside H-phosphonate is synthesized on a CPG resin via a succinyl linker as described above, and treated with a 0.2M solution of Beaucage reagent in BSA-CH₃CN (1:8, v/v) at RT for 30 min, and the resin is washed with CH₃CN. The resin is then treated with a 25% NH₃ aqueous solution at RT for 12 h, and washed with H₂O. The combined aqueous solutions are concentrated to dryness under reduced pressure, and the residue is analyzed and characterized by RP-HPLC and MALDI-TOF-MS. The RP-HPLC is performed with a linear gradient of 0-20% acetonitrile in 0.1M ammonium acetate buffer (pH 7.0) for 60 min at 50° C. at a flow rate of 0.5 mL/min using a μBondasphere 5 μm C18 column (100 Å, 3.9 mm×150 mm) (Waters).

Example 70

The corresponding oligonucleoside H-phosphonate is synthesized on a CPG resin via a succinyl linker as described above, and treated with a 0.2M solution of Beaucage reagent in BSA-CH₃CN (1:8, v/v) (0.2 mL) at RT for 30 min, and the resin is washed with CH₃CN. The resin is treated with a 25% NH₃ aqueous solution (5 mL) at RT for 24 h, and washed with H₂O. The combined aqueous solutions are concentrated to dryness under reduced pressure, and the residue is analyzed and characterized by RP-HPLC and MALDI-TOF-MS. The RP-HPLC is performed with a linear gradient of 0-20% acetonitrile in 0.1M ammonium acetate buffer (pH 7.0) for 80 min at 30° C. at a flow rate of 0.5 mL/min using a μBondasphere 5 μm C18 column (100 Å, 3.9 mm×150 mm) (Waters).