Low molecular weight (LMW), medium molecular weight (MMW) and high molecular weight (HMW) chitosan and human OPG protein (Recombinant Human OPG; PeproTech, Rocky Hill, New Jersey, USA) were used in this study. Tris buffer (5 mmol L−1, pH 7.5) and dimethyl sulfoxide (DMSO) (Fisher Scientific, Leics, UK) were used throughout the experiment. Normal, human periodontal ligament (NHPL) fibroblasts and normal, human osteoblasts were obtained from Lonza (Lonza Inc., Walkersville, MD, USA). Penicillin-streptomycin (Bioscience Ltd, Buckingham, UK), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) propidium iodide and acridin orange (Sigma-Aldrich, St. Louis, MO, USA) were also used.
Acridin orange
Manufactured by Merck Group
Sourced in United States
Acridin orange is a fluorescent dye used in various laboratory applications. It is a yellow-orange crystalline powder that emits orange-red fluorescence when exposed to ultraviolet or blue light. The dye is commonly used for staining and labeling biological samples, particularly for the detection and visualization of nucleic acids, such as DNA and RNA, in microscopy and flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Tris buffer, by Thermo Fisher Scientific (1 mentions)
Normal human osteoblasts, by Lonza (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt propidium iodide, by Merck Group (1 mentions)
Recombinant human opg, by Thermo Fisher Scientific (1 mentions)
Acridine orange solution, by Merck Group (1 mentions)
Acridine orange, by Merck Group (1 mentions)
3 protocols using acridin orange
1
Chitosan and Recombinant Human OPG on Periodontal Cells
2
Mitochondrial Health and Autophagy Evaluation
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JC1 staining: the membrane-permeant green fluorophore JC-1 (Thermofisher, Waltham, MA, USA) was used in apoptosis studies to evaluate mitochondrial health. JC-1 dye exhibits potential-dependent accumulation in mitochondria as red fluorescent JC1-aggregates. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio.
Acridin orange: the acridine orange solution (Sigma Aldrich, Burlington, MA, USA) was used in autophagy studies to evaluate acidic vesicles organelles formation. acridine orange solution is a cell-permeable green fluorophore that can be protonated and trapped in acidic vesicular organelles, where its accumulation promotes a red fluorescence emission. The presence of an orange/red staining is indicative of acidic vesicular organelles formation.
Acridin orange: the acridine orange solution (Sigma Aldrich, Burlington, MA, USA) was used in autophagy studies to evaluate acidic vesicles organelles formation. acridine orange solution is a cell-permeable green fluorophore that can be protonated and trapped in acidic vesicular organelles, where its accumulation promotes a red fluorescence emission. The presence of an orange/red staining is indicative of acidic vesicular organelles formation.
3
Acridine Orange Staining for Fluorescence Microscopy
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Acridin orange stained were performed as described previously.14 (link) Briefly, the Pa-PDT treated cells were stained with acridine orange (1 μg ⁄ ml; Sigma–Aldrich) for 15 min. The samples were washed twice with PBS and then examined under a fluorescence microscope (Opinity, China).
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