The collected ACM was used to quantify TNF, IL-6, IL-1β, and IFNγ using the MILLIPLEX MAP RAT cytokine/chemokine magnetic bead panel RECYTMAG-65K (Merck), following the manufacturer’s instructions. Data acquisition and analysis were performed in the LUMINEX platform using the Q-View (Quansys Biosciences) Software.
Q view
Manufactured by Quansys Biosciences
The Q-View is a multipurpose instrument designed for the quantitative analysis of biomolecules. It utilizes advanced imaging technology to capture and analyze data from various assays and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Recytmag 65k, by Merck Group (1 mentions)
Elisa kit, by InvivoGen (1 mentions)
Q plex array, by Quansys Biosciences (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
Q view imager, by Quansys Biosciences (1 mentions)
Quansys imager, by Quansys Biosciences (1 mentions)
Q plex technology, by Quansys Biosciences (1 mentions)
4 protocols using q view
1
Quantifying Rat Cytokine/Chemokine Levels
2
Plasma Cytokine and Chemokine Profiles
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Enzyme-linked immunosorbent assay (ELISA) kits (InvivoGen) were used to measure IFN-α and IFN-β in the plasma following the manufacturer’s instructions. The plates were scanned with FLUOstar Omega microplate reader. Limits of detection: IFN-α, 7.8–500 pg/ml and IFN-β, 15.6–1000 pg/ml. A Q-plex array (Quansys Biosciences) was used to determine cytokine and chemokine levels in plasma. The plate was scanned with Odyssey Infrared Imaging System (LI-COR Biosciences) and analyzed with Q-View (Quansys Biosciences). Limits of detection: TNF, 4.12–3000 pg/ml; IL-1β, 19.2–14000 pg/ml; IL-5, 6.86–5000 pg/ml; IL-6, 6.86–5000 pg/ml; IFN-γ, 10.97–8000 pg/ml; CCL1, 5.6–4000 pg/ml; CCL2, 4.12–3000 pg/ml; CCL22, 5.6–4000 pg/ml.
3
Multiplex Protein Quantification Assay
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Quantitative protein analysis was performed using a multiplex, antibody-based assay (Q-Plex Human Angiogenesis; Quansys Biosciences, Logan, Utah) in accordance with the manufacturer’s recommendations. A high-resolution image was acquired (Q-View Imager; Quansys Biosciences) and the protein concentration within each PL sample was determined by comparing chemiluminescent intensities with that of an eight-point standard curve (Q-View; Quansys Biosciences).
4
Cytokine and Chemokine Profiling in SUDV Response
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The levels of secreted cytokines and chemokines were analyzed as previously described [13 ]. Cytokine and chemokines IL-2, IL-4, IL-5, IP-10, IFN-γ, and TNF-α were measured in the culture medium of PBMCs following SUDV and SUDV proteins GP, NP, VP30 and VP40 stimulation using Q-Plex technology (Quansys Biosciences, Logan, Utah, USA) according to the manufacturer's instructions. Data were collected with a Quansys Imager (Quansys Biosciences) and results analyzed using Q-View (Quansys Biosciences).
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